中国农业科技导报2024，Vol.26Issue(4)：27-36,10.DOI:10.13304/j.nykjdb.2023.0125
藜麦CqGAI基因密码子偏好性与进化分析
Codon Bias and Evolution Analysis of CqGAI in Chenopodium quinoa
摘要
Abstract
To clarify the codon usage characteristics of CqGAI gene,the sequence of CqGAI gene was cloned.The codon usage preference of CqGAI gene was analyzed using CodonW,SPSS software and EMBOSS online program,and neutral mapping,ENC analysis and parity preference bias analysis were performed with GAI genes of 25 plants.The results showed that the length of coding sequence(CDS)of CqGAI gene was 1 782 bp,which encoded 593 amino acids,and contained the unique structural domains of DELLA gene family such as DELLA,TVHYNP,NLS,VHIID,LHR and RVER.The CqGAI gene could rapidly responded to gibberellines(GA),which played a key role in the GA signaling pathway.Codon bias analysis showed that ENC,CAI and GC content of CqGAI gene were 54.14,0.21 and 46.18%,respectively,with weak codon bias,preference ending with A/T and 27 high frequency codons.The clustering analyses showed that the CqGAI gene had the closest preference to Caryophyllales.The base composition and correlation analysis revealed that the codon preference of CqGAI gene was influenced by selection effect and base mutation.The frequency of codon usage indicated that both Escherichia coli and yeast were suitable for heterologous expression of CqGAI gene,and Arabidopsis thaliana,Nicotiana tabacum and Beta vulgaris were suitable for genetic transformation recipients for functional analysis of CqGAI gene.Above results provided an important reference for further study the function and heterologous expression of CqGAI gene.关键词
藜麦/CqGAI基因/密码子偏好性/进化分析Key words
Chenopodium quinoa/CqGAI gene/codon usage bias/evolution analysis分类
农业科技引用本文复制引用
丰扬,郭凤根,王仕玉,刘正杰,龙雯虹..藜麦CqGAI基因密码子偏好性与进化分析[J].中国农业科技导报,2024,26(4):27-36,10.基金项目
国家自然科学基金项目(31960417) （31960417）
云南省教育厅科学研究基金项目(2023Y1019). （2023Y1019）
