生物炭肥对易感根结线虫病烤烟根际菌群和理化性质的影响OA北大核心CSTPCD

In order to clarify the effect of biochar fertilizer on tobacco root knot nematode disease and rhizosphere soil,a field experiment was conducted to study the effect of different amounts of biochar fertilizer on rhizosphere soil of tobacco plant with root knot nematode disease.The results showed that the disease index of tobacco root knot nematode disease could significantly reduced by applying enzyme biocarbon fertilizer in the mixed pond when the amount of conventional fertilization was reduced by 10%.When 350 g·plant-1 enzyme biocarbon fertilizer was applied in the mixed pond,the control effect of tobacco root knot nematode disease was better,reaching 23.98%,the agronomic characters of tobacco plants were better,the activities of catalase,urease and phosphatase in rhizosphere soil were higher,the contents of phenolic acid in rhizosphere soil decreased,and allelopathy and autotoxicity weakened.The number of soil fungi in each treatment increased first and then decreased with the advance of growth period.When 350 g·plant-1 enzyme biocarbon fertilizer was applied,the bacterial number in the rhizosphere soil decreased gradually with the growth period.The number of fungi in the soil at each growth stage was high,the number of bacteria was low,and the value of fungi/bacteria was high.Above results indicated that,in tobacco planting,10%reduction in fertilization and 350 g·plant-1 application of enzyme biocarbon fertilizer in the pond could increase the number of fungi in the soil,reduce the number of bacteria in the soil,significantly improve soil fertility and enzyme activity,which provided scientific basis for biocarbon fertilizer to regulate the rhizosphere microecology of susceptible root knot nematodes.