中国人兽共患病学报2024，Vol.40Issue(3)：231-235,242,6.DOI:10.3969/j.issn.1002-2694.2024.00.043
乙型脑炎病毒部分NS1基因假病毒的构建
Construction of pseudoviruses containing a partial NS1 gene of Japanese encephalitis virus
摘要
Abstract
Construction of pseudoviruses containing a partial non-structural protein 1(NS1)gene of Japanese encephalitis virus(JEV)with nuclease resistance for application as quality control reagents in laboratory assay.Firstly,the plasmid pET-32a-MS2 underwent optimization and modification.Secondary,fragments of the partial NS1 gene of the JEV,along with inter-nal reference genes from mosquitoes and humans(mosquito 18S RNA and human GAPDH),were inserted into this plasmid,resulting in the construction of the recombinant plasmid pET-MS2-JEV.Subsequent steps involved the expression and purifica-tion of pseudoviruses.The construction of the recombinant plasmid pET-MS2-JEV yielded pseudoviruses,which obtained resil-ience against nucleases and various physical and chemical factors.These pseudoviruses exhibited stability when stored at various temperature conditions,including 4 ℃ and-20 ℃.This study accomplished the production of pseudoviruses characterized by outstanding stability,thus serving as reliable quality control reagents for JEV laboratory assays.关键词
乙型脑炎病毒/MS2噬菌体/假病毒Key words
Japanese encephalitis virus/bacteriophage MS2/pseudovirus分类
医药卫生引用本文复制引用
龙彩韵,聂凯,王环宇,王瑞晨,姚晓慧,武婕慧,付士红,李樊,殷启凯,崔倩倩,许松涛..乙型脑炎病毒部分NS1基因假病毒的构建[J].中国人兽共患病学报,2024,40(3):231-235,242,6.基金项目
国家重点研发计划(No.2020YFE0205700,No.2022YFC2302700) Supported by the National Key Research and Development Program(No.2020YFE0205700,No.2022YFC2302700) （No.2020YFE0205700,No.2022YFC2302700）
