中国水产科学2024，Vol.31Issue(2)：219-231,13.DOI:10.12264/JFSC2023-0306
珠江口万山群岛黄鳍棘鲷食性随年龄的变化特征
Diet change of the yellowfin sea bream(Acanthopagrus Iatus)with age from Wanshan Islands in the Pearl River Estuary
摘要
Abstract
Yellowfin sea bream(Acanthopagrus latus),an economically important fish and stock enhancement species,is distributed in the southeastern coastal areas of China.Study on diet change of yellowfin sea bream will contribute to the knowledge for the accurate food composition and ecological function of food wed for yellowfin sea bream.However,information on the feeding habits of wild yellowfin sea bream in natural marine environments is scarce.In this study,the feeding characteristics of yellowfin sea bream of different body lengths(1-3 years old)collected from the Wanshan Islands were analyzed based on stomach morphology and high-throughput sequencing of the 18S rRNA.Here,the 17 collected fishes were divided into three groups:the S group[body length:(194±13.1)mm],the M group[body length:(231±15.5)mm],and the L group[body length:(294±33.3)mm].Morphological identification showed that shellfish,crabs,and chyme were present in the stomach contents of the yellowfin sea bream.Most fish contained a high proportion of chyme.A total of 84 species of eukaryotes in 16 phyla were identified in the stomach contents of the yellowfin sea bream using high-throughput sequencing of the 18S rRNA gene.Therefore,18S rRNA high-throughput sequencing analysis provided a higher taxonomic resolution of the dietary composition than microscopic examination.Furthermore,high-throughput sequencing analyses showed that the major food sources of yellowfin sea bream were Charybdis feriatus and Centropages abdominalis of Arthropoda,Brachidontes variabilis of Mollusca,Nematalosa nasus of Chordata,and Neopyropia yezoensis of Rhodophyta,accounting for 66.73%of total food sources.Yellowfin sea bream in the Wanshan Islands mainly fed on animal food and feeding on plant food was relatively low.At the phylum level,the S group were dominant with Arthropoda,Mollusca and Rhodophyta,accounting for 93.88%of all sequences.Chordata,Cnidaria,and Arthropoda were the dominant phyla in the M group,accounting for 86.72%of all sequences,whereas L group was dominated by Chordata,Platyhelminthes,and Arthropoda,accounting for 89.25%of all sequences.Diet analysis of 1-3-year-old yellowfin sea bream revealed that with an increase in body length,the major feeding species of yellowfin sea bream gradually shifted from Arthropoda and Mollusca to Chordata,and the number of feeding species gradually decreased.Compared with that of the other Sparidae fishes in the Pearl River Estuary,a similar dietary habit was observed between Sparidae fishes,indicating feed-food competition for Sparidae fishes from the Pearl River Estuary.Sparidae are omnivorous and mostly consume benthos,plankton,and fish.These results provide basic data on the feeding ecology of yellowfin sea bream in the Wanshan Islands.关键词
黄鳍棘鲷/高通量测序技术/食性/胃含物/体长Key words
Acanthopagrus latus/high-throughput sequencing/diet/stomach content/body length分类
农业科技引用本文复制引用
杨禧越,吴鹏,刘永,李纯厚,唐广隆,张达娟,游奕来,潘淑芳,郑秋实,肖雅元..珠江口万山群岛黄鳍棘鲷食性随年龄的变化特征[J].中国水产科学,2024,31(2):219-231,13.基金项目
国家重点研发计划课题项目(2019YFD0901201,2019YFD0901204) （2019YFD0901201,2019YFD0901204）
农业农村部财政专项(NFZX2021) （NFZX2021）
广东省基础与应用基础研究基金项目(2022A1515010896) （2022A1515010896）
中央级公益性科研院所基本科研业务费项目(2023TD16) （2023TD16）
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项(2021SD04,2020SY01) （2021SD04,2020SY01）
珠海市社会发展领域科技计划项目(2320004343). （2320004343）
