中国输血杂志2024，Vol.37Issue(4)：377-384,8.DOI:10.13303/j.cjbt.issn.1004-549x.2024.04.002
USP9X对Akt磷酸化水平及血小板活化的影响
Effect of USP9X on Akt phosphorylation and platelet function
摘要
Abstract
Objective To explore the expression of USP9X in platelets and its effect on platelet function.Methods The expression of USP9X in human and mouse was evaluated by PCR and Western blot.Platelets from young and old mice were separated and prepared,and the expression of USP9X was detected.USP9X inhibitos were used to assess the regulation of USP9X in platelet function,including aggregation,ATP release and spreading.Platelet lysates were collected in different time points to evaluate the change of phosphorylation of Akt in USP9X inhibitors treated platelets.Results Both human and mouse platelets expressed USP9X.Compared to the young mice,the old mice showed significantly enhanced expression of USP9X(P<0.05).To assess the effect of USP9X on platelet function,USP9X inhibitor was used to pre-incubate platelets for 30 min and platelet function were examined later.Results showed that USP9X inhibitor significantly decreased platelet activation including aggregation,ATP release and spreading(P<0.05).Compared to the control group,the inhibitor treated group showed a significant decrease in the spreading area after 45 minutes.The Western blot results showed a significant de-crease in Akt phosphorylation levels of platelets in the USP9X inhibitor treated group.Conclusion Both human and mouse platelet express USP9X,and inhibition of USP9X decreased platelet function including aggregation,ATP release and sprea-ding.USP9X can also influence the phosphorylation of Akt.The inhibitor of USP9X may become a potential therapeutic tar-get for thrombosis intervention.关键词
USP9X/血小板/血栓/Akt磷酸化Key words
USP9X/platelet/thrombosis/Akt phosphorylation分类
医药卫生引用本文复制引用
贾雪梅,邵树军,周璐洁,杜丹心,卢煌莹,陈诚,夏荣..USP9X对Akt磷酸化水平及血小板活化的影响[J].中国输血杂志,2024,37(4):377-384,8.基金项目
国家自然科学基金面上项目(82070197) （82070197）
