间充质干细胞来源的外泌体治疗放射性肠炎的疗效与安全性OA北大核心CSTPCD

Objective:To explore the effects of mesenchymal stem cell-derived exosomes(MSC-Exo)on the in vitro proliferation and migration of human colorectal cancer cells(CT26),and assess the effects and safety of MSC-Exo treatment for tumor-bearing mouse model with radiation enteritis(RE).Methods:Commercially available liquid dressing products containing MSC-Exo(MSC-Exo product)were utilized for this study.Exosome components were identified through Nanoparticle Tracking Analysis(NTA),transmission electron microscopy(TEM),and WB analysis.The effects of MSC-Exo product on CT26 cell proliferation and migration were detected using the CCK-8 and Transwell assays.A CT26 tumor-bearing mouse model was established,and the mice were intragastrically administered with 400 μL MSC-Exo product,MSC culture medium,or saline for 7 consecutive days to evaluate the effects of MSC-Exo product on tumor growth and mouse survival in vivo.A radiation enteritis tumor-bearing mouse model was also established,and administered similar treatments.The efficacy and safety of MSC-Exo product treatment for RE were evaluated through H-E staining of small intestine tissues.Results:NTA,TEM,and WB analyses confirmed the presence of MSC-Exo in the commercial liquid dressing products.In vitro studies showed that MSC-Exo product did not promote tumor proliferation or migration.In vitro studies revealed that MSC-Exo product did not promote the proliferation and migration of colon cancer CT26 cells and was thus safe.In vivo studies revealed that gavage administration of MSC-Exo product did not affect tumor growth and the survival period of tumor-bearing mice.However,after treating radiation enteritis tumor-bearing mouse model with MSC-Exo product via gavage,the symptoms of bloody and mucous stool were relieved.H-E staining results demonstrated improved tissue morphology integrity in the intestinal tissues of the mice compared with that of the mice in the control group,suggesting that MSC-Exo product was effective in treating RE in tumor-bearing mice and was safe in terms of oncogenicity and tumor metastasis.Conclusion:Commercial liquid dressing products containing MSC-Exo do not promote the proliferation and migration of colon cancer cells in vitro,and have no obvious effects on tumor growth and survival period of CT26 cell tumor-bearing mice,but are effective in treating RE in the tumor-bearing mouse model and improve intestinal tissue damage.