玉米多叶矮化突变体lyd1的鉴定与基因克隆OA北大核心CSTPCD

The decrease of plant height in maize is usually caused by the decrease in the number of internodes,the shortening of internodes or the combination of both.However,in this study,the mutant leafy dwarf1(lyd1)found in the progeny of gene editing,exhibited more leaves and shorter stature.Quantitative measurements indicated the plant height of mutant lyd1 was only 93.10 cm,the plant height of wild-type KN5585 was 159.95 cm.The plant height was significantly reduced by 41.79%in mutant lyd1 com-pared with the wild type KN5585.The wild type KN5585 produced an average of 17.8 leaves at maturity stage,whereas mutants lyd1 produced 27.8 leaves.The number of leaves were significantly increased by 56.18%in mutant lyd1 compared with the wild type.Genetic analysis showed that the mutation phenotype of lyd1 was controlled by a pair of recessive nuclear genes.We applied a map-based cloning strategy to identify the gene responsible for the lyd1 phenotype.The gene was located between Indel10 and Indel11 on maize chromosome 3,and the physical distance was 0.74 Mb.Gene sequencing analysis of 13 genes(excluding pseu-dogenes)within the interval revealed that one base A was substituted in the fourth exon of ZmTE1,and there was no significant difference in other genes.ZmTE1 encoded an RNA-binding protein.The amino acid substitution was in the third RNA binding domain(RRM3),resulting in the conversion of aspartic acid to valine.The mutation sites of the mutant lyd1 were different from te1-mum1,te1-mum2,te1-mum3,and zm66 in previously reported.The discovery of lyd1 provides valuable materials for further analysis of the genetic mechanism of the balance between leaves and internodes development in maize.