中国现代中药2024，Vol.26Issue(3)：507-517,11.DOI:10.13313/j.issn.1673-4890.20230303002
基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定
Rapid Identification of Chemical Components in Composite Yizhihao Granules Based on UHPLC-Q-Exactive Orbitrap MS
摘要
Abstract
Objective:The chemical components in Composite Yizhihao Granules were analyzed using UHPLC-Q-Exactive Orbitrap MS.Methods:Thermo Scientific Syncronis C18 column(100 mm×2.1 mm,1.7 μm)was used with a mobile phase of 0.1%formic acid acetonitrile and a gradient elution at the flow rate of 0.3 mL·min-1.The column temperature was maintained at 45℃,and the injection volume was 2 μL.The mass spectrometry data of Composite Yizhihao Granules were collected in positive and negative ion mode.According to the accurate molecular weight and fragment ion information provided by the high-resolution mass spectrometry,the chemical components of Composite Yizhihao Granules were identified and analyzed in combination with the comparison of standard products and relevant literature reports.Results:A total of 90 compounds were identified and analyzed,including 2 amino acids,8 terpenes,25 organic acids,11 nitrogen-containing compounds,and 44 flavonoids.Among them,16 components were confirmed by comparison with the reference standards.Conclusion:In this study,the chemical components of Composite Yizhihao Granules were analyzed and identified,providing a basis for its quality control and the study of its pharmacological substances.关键词
复方一枝蒿颗粒/超高效液相色谱-四极杆-静电轨道场离子阱质谱法/化学成分Key words
Composite Yizhihao Granules/UHPLC-Q-Exactive Orbitrap MS/chemical components分类
医药卫生引用本文复制引用
刘明娟,李凯琳,张敏,唐苏女,刘玲,李晴,郭俐兰,杨建波,李辉..基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定[J].中国现代中药,2024,26(3):507-517,11.基金项目
新疆维吾尔自治区2023年度第二批"2+5"重点人才计划项目 （）
