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基于离子液体的菌藻类胡萝卜素提取工艺研究

张志平 宋洋洋 王秋领 王清福 龚贵平 付瑜锋 陈高

轻工学报2024,Vol.39Issue(2):19-27,9.
轻工学报2024,Vol.39Issue(2):19-27,9.DOI:10.12187/2024.02.003

基于离子液体的菌藻类胡萝卜素提取工艺研究

Research on extraction process of carotenoids from yeasts and algae based on ionic liquid

张志平 1宋洋洋 1王秋领 2王清福 2龚贵平 3付瑜锋 2陈高4

作者信息

  • 1. 郑州轻工业大学 食品与生物工程学院,河南 郑州 450001
  • 2. 河南中烟工业有限责任公司 技术中心,河南 郑州 450003
  • 3. 农业农村部成都沼气科学研究所,四川 成都 610042
  • 4. 山东省农业科学研究院,山东 济南 250100
  • 折叠

摘要

Abstract

Using Rhodotorula glutinis and Chlorella vularis sludge as raw material,the extraction process of carote-noids from yeast and algae was optimized through single factor and response experiments,and the in vitro antioxi-dant activity of carotenoids from yeast and algae was investigated.The results showed that the optimal ionic liquid for yeast and algal wall breaking was 1-butyl-3-methylimidazole chloride(BMIMCl),and the optimal extraction process for carotene from yeast and algal sludge was 1 mol/L ionic liquid concentration,pre-treatment temperature of 53℃,and pre-treatment time of 57 min.Under this condition,the extraction amount of carotene from yeast and algae was 1.311 mg/g.When the mass concentration was 30 μg/mL,the scavenging rates of DPPH free radical,·OH free radical and ABTS+ free radical were 82.3%,37.6%and 86.8%,respectively,and the reducing power was 0.289,showing good antioxidant activity.The yeast and algal sludge treated with BMIMCl had a higher content of extracted carotenoids and better antioxidant activity.

关键词

离子液体/菌藻泥/类胡萝卜素/提取工艺/抗氧化活性

Key words

ionic liquid/yeast and algal sludge/carotenoids/extraction process/antioxidant activity

分类

轻工纺织

引用本文复制引用

张志平,宋洋洋,王秋领,王清福,龚贵平,付瑜锋,陈高..基于离子液体的菌藻类胡萝卜素提取工艺研究[J].轻工学报,2024,39(2):19-27,9.

基金项目

河南省科技攻关项目(212102310077) (212102310077)

中原科技创新领军人才项目(224200510017) (224200510017)

轻工学报

OA北大核心

2095-476X

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