轻工学报2024，Vol.39Issue(2)：36-42,7.DOI:10.12187/2024.02.005
韭花精油主成分对单增李斯特氏菌的抑菌活性和抑菌机理
Antibacterial activities and mechanisms of the major compositions of Allium tenuissimum flower essential oil against Listeria monocytogenes
摘要
Abstract
This study investigated the antibacterial activities and mechanisms of the major compositions(allyl methyl disulfide and dimethyl trisulfide)of Allium tenuissimum flower essential oil against Listeria monocytogenes.The antibacterial activities and mechanisms of these two compositions against L.monocytogenes were evaluated by minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),cellular morphologies,etc.These results showed that MICs of these two compositions were both 1.0 mg/mL,and MBCs were both 2.0 mg/mL.The 2.0 mg/mL of allyl methyl disulfide and dimethyl trisulfide rigorously impaired cellular morpholo-gies and cytomembrane structures of L.monocytogenes.The leakage of β-galactosidases and proteins significantly increased when bacterial cells were treated with these two compositions at 4.0 mg/mL.ATPase activities(0.52 U/mg prot and 0.55 U/mg prot)of the cells treated with allyl methyl disulfide(4.0 mg/mL)and dimethyl trisulfide(4.0 mg/mL)were distinctly lower than the activity of control(3.05 U/mg prot,P<0.05),respectively.Fur-thermore,the contents of DNA for bacterial cells were remarkably decreased after being treated with these two com-positions.Thus,allyl methyl disulfide and dimethyl trisulfide inhibited activities of L.monocytogenes via destruction of cytomembrane structures,inactivation of ATPase and reduction of DNA contents in bacterial cells.关键词
细叶韭花/精油/烯丙基甲基二硫醚/二甲基三硫醚/单增李斯特氏菌/抑菌活性Key words
Allium tenuissimum flower/essential oil/allyl methyl disulfide/dimethyl trisulfide/Listeria monocyto-genes/antibacterial activity分类
轻工纺织引用本文复制引用
吴澄宇,李迎秋..韭花精油主成分对单增李斯特氏菌的抑菌活性和抑菌机理[J].轻工学报,2024,39(2):36-42,7.基金项目
山东省重点研发计划项目(2021LYXZ018,2020CXGC010604) （2021LYXZ018,2020CXGC010604）
齐鲁工业大学(山东省科学院)科教产融合试点工程重大创新专项项目(2022JBZ01-08) （山东省科学院）
