BPGM下调对静原鸡成肌细胞生长发育的影响OA北大核心CSTPCD

The aim of this study was to investigate the effect of bisphosphoglycerate mutase(BPGM)on the growth and development of chicken myogenic cells.The transcriptomics and proteomics data was jointly analyzed in the preliminary stage of the study to identify the differentially expressed genes that might affect the growth and de-velopment,and investigated their effects on the proliferation,apoptosis and expression levels of related marker genes of myogenic cells.Inosinic acid(IMP)was detected by ELISA and adenosine triphosphate(ATP)was detected by colorimetric assay,which revealed the important roles of the key candidate genes on muscle development and meat flavor of Jingyuan chickens.The results showed that a total of 139 differentially expressed genes/proteins were iden-tified between the breast muscle and leg muscle of Jingyuan chickens,including 75 differentially expressed genes/proteins that were up-regulated in both the transcriptome and proteome,45 differentially expressed genes/proteins that were down-regulated in both the transcriptome and proteome,13 differentially expressed genes/proteins that were down-regulated in the transcriptome,and 6 differentially expressed genes/proteins that were up-regulated in the proteome and down-regulated in the transcriptome.7 differentially expressed genes were screened from the gly-colysis/glycolysis pathway,which were BPGM,phosphoglycerate kinase 1 gene(PGK1),glucose-6-phosphate isomerase gene(GPI),phosphoglycerate mutase 1 gene(PGAM1),lactate dehydrogenase A gene(LDHA),lactate dehydrogenase B gene(LDHB)and phosphoglycerate isomerase 1 gene(TPI1),and screened for the differentially expressed gene BPGM in combination with KEGG pathway enrichment analysis and protein network interactions a-nalysis.BPGM was expressed in all tissues of Jingyuan hens,the highest expression was in pectoral muscle,and it showed an up-regulation trend during the differentiation of myoblasts.Primary myogenesis cells were isolated and cultured for cell transfection.Interfering with BPGM inhibited myoblast proliferation and promoted apoptosis;it also affected genes upstream and downstream of the glycolytic pathway,and inhibited the biosynthesis of IMP and ATP at the same time.In conclusion,down-regulation of BPGM inhibited the growth and development of myoblasts,as well as the biosynthesis of IMP and ATP.