海南医学院学报2024，Vol.30Issue(8)：597-606,10.DOI:10.13210/j.cnki.jhmu.20231129.002
基于网络药理学和实验验证探讨麦门冬汤治疗特发性肺纤维化的作用机制
Mechanism of Maimendong decoction in treating idiopathic pulmonary fibrosis based on network pharmacology and experimental validation
摘要
Abstract
Objective:To investigate the therapeutic effect and mechanism of Maimendong decoction on idiopathic pulmonary fibrosis by utilizing network pharmacology methods and combining experimental research.Methods:TCMSP,ETCM and Swiss ADME were used to screen the active ingredients of Maimendong decoction.Through PubChem,SwissTargetPrediction and Uni-port databases,the action targets of various components of traditional Chinese medicine were screened.The GeneCards database and OMIN database were applied to obtain target genes of idiopathic pulmonary fibrosis.Venn diagrams drawing tool was used to screen the intersection targets of traditional Chinese medicine and diseases.The"traditional Chinese medicine active ingredient dis-ease target"network diagram was constructed by using the Cytoscape 3.9.1 software,and the protein interaction network(PPI)was constructed by using the CytoNCA plug-in to screen the core target proteins.DAVID was used for enrichment analysis of GO and KEGG pathways.Finally,we used bleomycin to establish a pulmonary fibrosis model in SD rats,observed the macroscopic specimens and pathological sections of rat lung tissue,and conducted real-time fluorescence quantitative PCR analysis to compare the mRNA expression levels of TGF-β1,α-SMA,IL-4,IFN-γ,LC3-Ⅱ,and beclin1 between the treatment group and the con-trol group.Results:A total of 402 therapeutic targets of Maimendong decoction for IPF were screened,including 50 core targets,corresponding to 159 active ingredients of 5 traditional Chinese medicines.GO enrichment analysis and KEGG enrichment analysis involved the PI3K-AKT signal pathway,MAPK signal pathway and other signal pathways to treat idiopathic pulmonary fibrosis.The animal experiment results showed that compared with the model group,the Maimendong decoction group could significantly reduce the relative expression level of TGF-β1,α-SMA and IL-4 mRNA(P<0.05),significantly upregulated the relative expres-sion level of mRNA LC3-Ⅱ,beclin1,IFN-γ(P<0.05).Conclusion:This study suggests that the key active components of Mai-mendong decoction may play a role in biological processes such as autophagy,inflammatory reaction,macrophage polarization,and improve pulmonary fibrosis.关键词
麦门冬汤/特发性肺纤维化/网络药理学/实验验证/自噬Key words
Maimendong tang/Idiopathic pulmonary fibrosis/Network pharmacology/Experiment validation/Autophagy分类
医药卫生引用本文复制引用
马先,于明玥,周博文,李泽峰,李传慧,辛玲杰,贾新华..基于网络药理学和实验验证探讨麦门冬汤治疗特发性肺纤维化的作用机制[J].海南医学院学报,2024,30(8):597-606,10.基金项目
This study was supported by Qilu Hygiene and Health Leading Talent Cultivation Project[Luwei Talent Zi(2020)No.3] （2020）
Shandong Provincial Natural Science Foundation General Project(ZR2020MH353) （ZR2020MH353）
2022 Youth Qihuang Scholar Training Program[Guozhong Pharmaceutical Education Letter(2022)No.256] （2022）
Shandong Province Traditional Chinese Medicine High Level Talent Cultivation Project[Lu Wei Han(2023)No.143] 齐鲁卫生与健康领军人才培育工程[鲁卫人才字(2020)3号] （2023）
山东省自然科学基金面上项目(ZR2020MH353) （ZR2020MH353）
2022年青年岐黄学者培养项目[国中医药人教函(2022)256号] （2022）
山东省中医药高层次人才培育项目[鲁卫函(2023)143号] （2023）
