陕西部分地区长角血蜱的鉴定及其基因多态性OA北大核心CSTPCD

This experiment was conducted to compare the differences in ribosome genes of Haemaphysalis longicornis,which collected from Weinan city and Shangluo city,Shaanxi province,and explore appropriate molecular markers to provide a foundation for Haemaphysalis longicornis identification.Morphological structures of ticks were observed by stereomicroscope;the ribosome 18S,ITS1,5.8S,ITS2 and 28S sequences of Haemaphysalis longicornis were determined by molecular biology techniques,the homology of genes was compared,and the phylogenetic tree was constructed to analyze the gene polymorphism.The results showed that 5.8S sequence of ribosome could not distinguish ticks from different species,while 18S,28S,ITS1 and ITS2 sequences could distinguish ticks from different species.Though 18S and ITS1 sequences could distinguish Haemaphysalis longicornis from China and overseas,Haemaphysalis longicornis from different regions in China could be distinguished by the 18S sequence.By comparing the ribosome gene fragments of Haemaphysalis longicornis in Shaanxi province,the phylogenetic relationship showed that the 18S sequence has the strongest ability to distinguish different region strains of Haemaphysalis longicornis and could be used as an effective tool for the classification and molecular identification of Haemaphysalis longicornis.