猪源葡萄球菌的分离鉴定及耐药性分析OA北大核心CSTPCD

To clarify the pathogenic bacteria isolated from a large-scale pig farm in Fujian,their biological characteristics,pathogenicity,and resistance to Chinese and western medicines were conducted to provide reference basis for the prevention and control of diseases.In the test,the isolated bacteria were purified,and the biological characteristics,resistance to Chinese and western medicines and other related tests of the isolated bacteria were carried out by Gram stain,PCR identification,drug sensitivity test,disinfectant disinfection and sterilization test,drug resistance gene test and animal challenge test.The results showed that the isolated bacteria were Staphylococcus.The bacterium is highly sensitive to 14 of the 30 commonly used western antimicrobials,including oxacillin,norfloxacin and cephalosporins.It is moderately sensitive to 5 drugs,including amikacin,gentamicin,tetracycline,vancomycin and florfenicol.It is not sensitive to 11 drugs,including ampicillin,piperacillin and kanamycin.The detection of six resistance genes,including cfr,tetM,floR,mcr-1,blaNDM-1 and optrA,is nega-tive;The bacterium is highly sensitive to tannic acid in 26 kinds of traditional Chinese medicines,moderately sensi-tive to Schisandra chinensis and Wuxinzi polyphenols,low sensitive to Yexiazhu,Magnolia officinalis,Polygonum cuspidatum,and insensitive to 20 kinds of traditional Chinese medicines,such as Lobelia chinensis lobata,iron amaranth,wormwood leaf,Paris polyphylla;Among the eight disinfectants,84 disinfectant had poor disin-fection and sterilization effect on the isolated bacteria,while three disinfectants,namely bromogeramine,trimethylammonium chloride and compound phenol,had good disinfection and sterilization effect on the isolated bacteria;The animal challenge test showed that the bacterium was highly pathogenic to 25 day old healthy weaned piglets,and could lead to typical clinical symptoms and pathological characteristics of staphylococcosis in pigs.The histopathology showed congestion of the spleen red pulp,exudation of red blood cells in the alveolar cavity,and a small amount of powder stained exudate.The research aims to isolate pig derived Staphylococcus aureus and clarify its pathogenicity and drug resistance in the context of prohibiting the addition of antibiotics in the feed,providing theoretical reference and technical support for the prevention and treatment of pig staphylococcosis.