鸡后口吸虫的形态学特征及分子鉴定OA北大核心CSTPCD

The presence of trematodes in chicken ceca has been reported in many places,but there are few studies on its morphological and molecular identification.In Jinzhou of Liaoning district,a few trematodes were isolated from chicken ceca.In order to identify the species of several chicken trematodes isolated from Jinzhou,morphological observations of adults and eggs were carried out.Observations showed that the trematode is tongue-shaped with a well-developed oral sucker and pharynx,short esophagus,and two curved intestinal branches that extend to the end of the body.The testes are arranged in the back of the body,the ovary is located on the opposite side of the testes,and the vitellaria gland is well developed,and extended to the front of the worm's body.While the egg of Postharmostomum gallinum with a color of light yellow,looks like the sesame,has the structure of egg lip and contains a mature miracidum.The trematode was identified as Postharmostomum gallinum after morphological observation.PCR was used to amplify the rDNA ITS sequence of the fluke.After sequencing,NCBI database was used to compare and analyze the homology between the sequenced sequence and the sequence of Postharmostomum gallinum from chickens in Hunan with the MH915391,the homology was 99.76％.Phylogenetic tree was construc-ted with ITS-2 sequences.Results showed that the trematode isolated in this study and Postharmostomum gallinum from Hunan were clustered in one branch,and provided a reference for further molecular diagnosis of Postharmostomum.