鸭疫里默氏菌噬菌体宿主谱的拓宽OA北大核心CSTPCD

To establish a method for broadening host range of Riemerella anatipestifer,a mixture of 6 phages was cultured with 6 strains of host bacteria and 18 strains of multi resistant Riemerella anatipestifer(which could not be cleaved by any of the six phages)respectively.The phages in the culture medium were harvested and mixed and cultured for 30 generations using the same method.The bacteriophage spectrum of 30 generations and the single phage clone in the 30 generations were measured by plaque analysis.The result showed that 79.2％(19/24)of the experimental strains of Riemerella anatipestifer could be lysed by the mixture of 30 generations.The number of test strains that could be lysed by the single phage isolated from the mixture of 30 generations was 2-9.The five single phages with the highest number of cleavages were subcultured 10 times with their respective host bacteria,and the bacteriophage spectrum did not change.19 strains of the experimental strains of Riemerella anatipestifer could be lysed by the mixture of 4 strains of single phage with complementary lysis characteristics,the same as the mixture of 30 generations.In conclusion,the established method can broaden the phage spectrum of phage mixtures and single phage,laying a foundation for the selection of strains of bacteriophage cocktail preparations.