PM2.5和热/冷暴露对小鼠骨骼肌和白色脂肪组织AKT/GLUT4通路的影响OA北大核心CSTPCD

[Background]It is unclear if there is any combined effect of air pollutants and non-optimal tem-perature on metabolic syndrome,or any molecular mechanisms of related signaling pathways in the process,which requires urgent systematic research. [Objective]To observe the effects of combined exposure to PM2.5 and non-optimal temperature on metabolic damage at gene and protein levels in mice,and elucidate the role of related signaling pathway in crucial organs. [Methods]A total of 60 six-week-old male C57BL/6J mice were randomly divided into six groups:a normal temperature-filter air group(TN-FA),a normal temperature-concentrated PM2.5 group(TN-PM),a heat-filter air group(TH-FA),a heat-concentrated PM2.5 group(TH-PM),a cold-filter air group(TC-FA),and a cold-concentrated PM2.5 group(TC-PM).The Shanghai Meteorological and Environmental Animal Exposure System(Shanghai-METAS)was used to provide combined exposure settings of air types[concentrated PM2.5 and filter air(FA)]and temperatures[normal(22℃),cold(4℃),and heat(30℃)]for 4 weeks.Skeletal muscle and white adipose tissue(WAT)of the mice were sampled at the end of exposure,and transcriptomics and Western blot(WB)assay were adopted to observe selected gene and protein expression levels in the samples respectively. [Results]The transcriptomics results indicated that the PM2.5 exposure enhanced the number of differentially expressed genes.Specifi-cally,4820 genes were differentially expressed in the TN-PM mice compared to the TN-FA mice at normal temperature,and 1143 genes were differentially expressed in the Tc-PM mice compared to the Tc-FA mice in the cold environment.The phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signaling pathway and the endoplasmic reticulum protein processing pathway were identified as the most significant pathways in metabolic injury resulting from combined exposure to PM2.5 and non-optimal temperature exposure.The WB results showed that exposure to PM2.5 in the normal temperature and the cold environments led to a significant increase in the expression of p-AKT in WAT(P<0.01,P<0.05)and a significant decrease in the expression of GLUT4(P<0.05,P<0.01).In skeletal muscle,exposure to PM2.5 led to a significant decrease in GLUT4(P<0.05)in all environments,with a consistent trend of change as observed in WAT. [Conclusion]Cold/heat exposure might promote PM2.5-induced metabolic disorder through suppression of the AKT/GLUT4 pathway,ag-gravating metabolic damage.