农业生物技术学报2024，Vol.32Issue(5)：1020-1036,17.DOI:10.3969/j.issn.1674-7968.2024.05.004
葡萄纤维素合酶超家族基因鉴定及其表达分析
Identification and Expression Analysis of Cellulose Synthase/Cellulose Synthase-like(Cesa/Csl)Superfamily Genes in Grape(Vitis vinifera)
摘要
Abstract
Fruit firmness is one of the important indicators of the quality of fresh grapes(Vitis vinifera),which greatly affects the commercial and market value of grapes.The structure and composition of cell walls are important factors leading to differences in grape fruit hardness.The cellulose synthase/cellulose synthase like(CesA/Csl)superfamily is a key enzyme involved in the synthesis of cellulose and hemicellulose in plant cell walls,which has a potential impact on fruit hardness.However,the grape CesA/Csl superfamily has not been systematically identified.In this study,to investigate the expression patterns of the grape CesA/Csl superfamily in different hard and soft meat grape varieties,bioinformatics methods were used to identify the genes of the grape CesA/Csl superfamily at the whole genome level.A phylogenetic evolution tree was constructed and the physicochemical properties,gene structure,and variety expression characteristics of the genes were analyzed.The results showed that there were 67 members of the grape CesA/Csl family,which were unevenly distributed on 15 chromosomes;Phylogenetic tree analysis showed that grape CesA/Csl members were divided into 6 subgroups,which were relatively conservative in evolution;The proteins encoded by members of this family were mainly distributed on the plasma membrane,with an amino acid length of 128～1 406 aa;The transcriptome analysis results showed that there were significantly differentially expressed CesA/Csl genes in the mature fruits of hard and soft meat grapes,which might be a key gene leading to differences in fruit hardness.In addition,there were significant differences in the expression of VvCesA4 and VvCslB5 at different developmental stages of the same variety,which might regulate the hardness changes during fruit growth and development.Through qRT-PCR and correlation analysis of fruit hardness among different varieties,it was found that VvCesA4 may negatively regulate fruit hardness,while VvCesA2 and VvCesA6 might positively regulate fruit firmness.Based on the above results,it could be found that the CesA/Csl superfamily plays different regulatory roles in grape hardness differences.This study provide theoretical basis for further exploring the mechanism by which the CesA/Csl superfamily regulates grape fruit firmness.关键词
纤维素合酶(CesA/Csl)基因/生物信息学/基因表达/果实硬度/葡萄Key words
Cellulose synthase/cellulose synthase-like(CesA/Csl)gene/Bioinformatics/Gene expression/Fruit firmness/Grapes分类
农业科技引用本文复制引用
胡玲玲,徐涛,杨杰,龚丽丽,杨语心,杨中义,吴月燕..葡萄纤维素合酶超家族基因鉴定及其表达分析[J].农业生物技术学报,2024,32(5):1020-1036,17.基金项目
宁波市科技创新2025现代种业重大专项(2019B10015) （2019B10015）
浙江省重点研发计划(2021C02053) （2021C02053）
浙江省教育厅一般科研项目(Y202351595) （Y202351595）
浙江省"生物工程"一流学科学生创新计划(CX2022025) （CX2022025）
浙江省大学生科技创新活动计划暨新苗人才计划(2023R419016) （2023R419016）
