刺叶高山栎(Quercus spinosa)内生真菌筛选鉴定及促生作用测定OA北大核心CSTPCD

Quercus spinosa is an evergreen tree or shrub of Quercus alpine group in Fagaceae,which has strong adaptability to the extreme ecological environment.To explore the growth-promoting function of endo-phytic fungi,the samples of roots,stems and leaves of Quercus spinosa were collected from Kunming City,Yunnan Province,and culturable endophytic fungi were isolated by tissue separation method.The obtained en-dophytic fungi were tested for their growth-promoting characteristics such as nitrogen fixation,phosphorus dis-solution,potassium dissolution and indole-acetic acid(IAA)production in order to select the strains with di-verse growth-promoting characteristics,and then the molecular biological and morphological identification were conducted,and their growth-promoting effects on seed germination and potted seedling growth of Lolium pe-renne were determined.The results showed that 74 strains of endophytic fungi were isolated from the roots,stems and leaves,and 11 of them were selected for testing growth-promoting characteristics.The results showed that 10 strains had IAA-producing ability,8 strains had nitrogen-fixing ability,one strain(JG2)possessed phosphorus-dissolving,potassium-dissolving and IAA-producing abilities,and 2 strains(J06 and JJ15)had nitrogen-fixing and good IAA-producing abilities.Through the molecular biological and morphological identifi-cation,JG2 was identified as Talaromyces erythromellis,while J06 as Bionectria ochroleuca,and JJ15 as Daldi-nia eschscholtzii.In the seed germination and pot experiments,the strain JG2 had significant growth-promoting effects on Lolium perenne seedlings,which showed that it had the potential to promote growth.