山东农业科学2024，Vol.56Issue(4)：133-144,12.DOI:10.14083/j.issn.1001-4942.2024.04.017
刺叶高山栎(Quercus spinosa)内生真菌筛选鉴定及促生作用测定
Screening and Identification of Endophytic Fungi from Quercus spinosa and Determination of Their Growth-Promoting Functions
摘要
Abstract
Quercus spinosa is an evergreen tree or shrub of Quercus alpine group in Fagaceae,which has strong adaptability to the extreme ecological environment.To explore the growth-promoting function of endo-phytic fungi,the samples of roots,stems and leaves of Quercus spinosa were collected from Kunming City,Yunnan Province,and culturable endophytic fungi were isolated by tissue separation method.The obtained en-dophytic fungi were tested for their growth-promoting characteristics such as nitrogen fixation,phosphorus dis-solution,potassium dissolution and indole-acetic acid(IAA)production in order to select the strains with di-verse growth-promoting characteristics,and then the molecular biological and morphological identification were conducted,and their growth-promoting effects on seed germination and potted seedling growth of Lolium pe-renne were determined.The results showed that 74 strains of endophytic fungi were isolated from the roots,stems and leaves,and 11 of them were selected for testing growth-promoting characteristics.The results showed that 10 strains had IAA-producing ability,8 strains had nitrogen-fixing ability,one strain(JG2)possessed phosphorus-dissolving,potassium-dissolving and IAA-producing abilities,and 2 strains(J06 and JJ15)had nitrogen-fixing and good IAA-producing abilities.Through the molecular biological and morphological identifi-cation,JG2 was identified as Talaromyces erythromellis,while J06 as Bionectria ochroleuca,and JJ15 as Daldi-nia eschscholtzii.In the seed germination and pot experiments,the strain JG2 had significant growth-promoting effects on Lolium perenne seedlings,which showed that it had the potential to promote growth.关键词
刺叶高山栎/内生真菌/分子生物学鉴定/形态学鉴定/促生特性Key words
Quercus spinose/Endophytic fungi/Molecular biological identification/Morphological identi-fication/Growth-promoting characteristics分类
农业科技引用本文复制引用
杨明国,芦俊佳,田芸菁,张梓月,刘勉,勾朝金,张倩婷,古旭,杨发忠..刺叶高山栎(Quercus spinosa)内生真菌筛选鉴定及促生作用测定[J].山东农业科学,2024,56(4):133-144,12.基金项目
国家自然科学基金项目(32060695) （32060695）
云南省青年人才(培养)项目(990122074) （培养）
云南省农业基础研究联合专项"粉红粘帚霉对楚雄腮扁叶蜂致病机理研究"(202301BD070001-091) （202301BD070001-091）
