食品工业科技2024，Vol.45Issue(10)：17-24,8.DOI:10.13386/j.issn1002-0306.2023100088
亚临界水萃取薄荷黄酮工艺优化及神经保护活性研究
Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq.with Subcritical Water
摘要
Abstract
Objective:To optimize the subcritical water extraction process for flavonoids from Mentha haplocalyx Briq.(mint)and assess the mitigating effects of peppermint flavonoids on H2O2-induced adrenal pheochromocytoma(PC12 cell)injury in rats.Methods:The subcritical water extraction process for mint flavonoids was optimized using single-factor and Box-Behnken response surface tests.A comparison was made with reflux extraction,ultrasonic-assisted extraction,and supercritical CO2 extraction methods.In vitro cell culture of PC12 cells was employed to establish an H2O2-induced PC12 cell injury model.The neuroprotective activity of mint flavonoids was analyzed using the MTT(methyl thiazolye tetrazolium,MTT)method.Results:The optimized extraction conditions were determined to be 142℃,38 min,and 21:1 mL/g,resulting in a mint flavonoid yield of 14.07%±0.23%.This yield was significantly higher(P<0.001)than that of reflux extraction(334%increase),ultrasonic-assisted extraction(75%increase),and supercritical CO2 extraction(173%increase).Mint flavonoids,within the concentration range of 1～100 μg/mL,significantly enhanced the survival rate of PC12 cells from 53.61%±0.64%to 78.49%±0.84%compared to the H2O2 group(P<0.001),indicating substantial alleviation of cellular oxidative injury.Conclusion:The study established an environmentally friendly,efficient,and reliable subcritical water extraction process for mint flavonoids.The extracted mint flavonoids demonstrated significant protective effects against H2O2-induced PC12 cell injury(P<0.01).关键词
薄荷黄酮/亚临界水萃取/响应面法/细胞损伤/神经保护活性/工艺优化Key words
Mentha haplocalyx flavonoids/subcritical water extraction/response surface methodology/cell injury/neuroprotective activity/process optimization分类
轻工纺织引用本文复制引用
张晴晴,张梦娇,马翊铭,蔡学一..亚临界水萃取薄荷黄酮工艺优化及神经保护活性研究[J].食品工业科技,2024,45(10):17-24,8.基金项目
安徽省优秀青年人才支持计划项目(gxyq2020070). （gxyq2020070）
