食品工业科技2024，Vol.45Issue(10)：93-100,8.DOI:10.13386/j.issn1002-0306.2023070209
酪蛋白磷酸肽-钙螯合物的分级、表征及持钙特性
Fractionation,Characterization and Calcium-holding Properties of Casein Phosphopeptide-Calcium Chelates
摘要
Abstract
In this paper,a novel casein phosphopeptide fractionation process was proposed based on the calcium chelating properties of casein phosphopeptide and different casein phosphopeptide fractions with different chelating abilities and enhanced purity were produced using different calcium ion concentrations.Three casein phosphopeptide-calcium chelate fractions(CPP1-Ca,CPP2-Ca and CPP3-Ca)were precipitated hierarchically from the trypsin digest of casein by increasing the Ca2+ addition step by step in an ethanol solution with a concentration of 55%(v/v),and PBS was used to decalcify to obtain the corresponding casein phosphopeptides(CPP1,CPP2 and CPP3).The physicochemical properties and calcium uptake characteristics of the three casein phosphopeptide-calcium chelate fractions were analyzed and compared with those of the peptide-calcium chelate fraction(CPP-Ca)obtained by the conventional calcium-ethanol precipitation method as a control.The results showed that among the three casein phosphopeptide-calcium chelate fractions,CPP3-Ca contained the highest purity and calcium chelating amount of 89.42%and 69.59 mg/g,respectively,which were significantly higher than those of the ungraded CPP-Ca of 83.77%and 55.05 mg/g.The analysis of the amino acid composition and the N/P(molar ratio)revealed that the CPP3-Ca contained a higher phosphatidylserine group,which in turn binds more Ca2+.Analysis of the calcium holding capacity of different casein phosphopeptide fractions showed that CPP3 was able to better delay and prevent the formation of calcium phosphate precipitation,and had a higher calcium holding capacity.Meanwhile,CPP3-Ca had higher calcium solubility in the simulated intestinal environment,indicating that the in vitro bioavailability of the graded casein phosphopeptide-calcium chelates was improved.关键词
钙乙醇分级沉淀/肽钙螯合物/酪蛋白磷酸肽/结构表征/持钙能力Key words
calcium-ethanol graded precipitation/peptide-calcium chelate/casein phosphopeptide/structural characterization/calcium-holding capacity分类
轻工纺织引用本文复制引用
刘卫震,王冠华,袁延佩,伍昌军,贾永秀,隋文杰..酪蛋白磷酸肽-钙螯合物的分级、表征及持钙特性[J].食品工业科技,2024,45(10):93-100,8.基金项目
天津市青年人才托举工程项目(TJSQNTJ-2020-05). （TJSQNTJ-2020-05）
