食品工业科技2024，Vol.45Issue(10)：158-164,7.DOI:10.13386/j.issn1002-0306.2023060010
小麦B淀粉一步酶解产糖优化及酒精发酵
Optimization of One-step Enzymatic Sugar Production from Wheat B Starch and Ethanol Fermentation
摘要
Abstract
Using wheat B starch as raw material,optimize the one-step enzymatic hydrolysis process for preparing fermentable sugars and subsequent fermentation of alcohol by brewing yeast to improve its utilization value.Initially,the elemental composition of Wheat B starch was quantified.A composite enzymatic catalyst containing α-amylase and saccharase were then employed to generate fermentable sugars from wheat B starch through enzymatic hydrolysis efficiently.Various parameters,including composite enzyme concentration,solid-liquid ratio,enzyme temperature,enzymatic duration,and pH were thoroughly investigated to assess their effects on reducing sugar yield.An orthogonal optimization approach was conducted to determine the optimal conditions for enzymatic hydrolysis.Results showed that wheat B starch contained 85.61%starch,with approximately 2%protein and mineral content,and pentosan composition nearing 5%.Following the orthogonal optimization,the most effective parameters for enzymatic saccharification were identified:A composite enzyme dosage of 4 mL,a solid-liquid ratio of 1:25 g/mL,an enzymatic digestion temperature of 60℃,an enzymatic digestion duration of 10 h,and a pH of 5.0.Under these specific conditions,the yield of reducing sugar reached 94.6%.Subsequently,wheat B starch was converted into alcohol through yeast-mediated fermentation.This process led to a final alcohol concentration 12.76 g/L,accompanied by an alcohol yield of 31.90%.In conclusion,wheat B starch holded remarkable promise for large-scale bioethanol production,providing valuable insights for maximizing the utilization of this resource on a broad scale.关键词
小麦B淀粉/一步酶解/正交优化/酒精发酵Key words
wheat B starch/one-step enzyme digestion/orthogonal optimization/ethanol fermentation分类
轻工纺织引用本文复制引用
张晶晶,惠明,陈家新,冯军伟,胡景婷,马欣荣,张楠,解心月,刘娜,王心..小麦B淀粉一步酶解产糖优化及酒精发酵[J].食品工业科技,2024,45(10):158-164,7.基金项目
河南省高等学校重点科研项目(22A180015) （22A180015）
河南省重点研发与推广专项(科技攻关)项目(232102310302) （科技攻关）
河南工业大学小麦和玉米深加工国家工程研究中心开放课题(NL2022015) （NL2022015）
许昌学院"揭榜挂帅"课题(2022JBGS06). （2022JBGS06）
