食品科学2024，Vol.45Issue(9)：44-50,7.DOI:10.7506/spkx1002-6630-20230906-050
6-姜烯酚玉米醇溶蛋白纳米颗粒的构建及其生物利用度分析
Construction and Bioavailability Analysis of 6-Shogaol-Loaded Zein Nanoparticles
摘要
Abstract
In order to solve the problem of the low bioavailability of 6-shogaol(6S),in this study,zein-sodium caseinate nanoparticles(ZCP)were constructed by the anti-solvent precipitation method as a nano-delivery carrier for 6S.The physicochemical properties of 6S-loaded zein nanoparticles(ZCP-6S)were characterized and the bioavailability was investigated by in vitro simulated digestion,Caco-2 cell model and pharmacokinetic studies in rats.The particle size distribution,microstructure and chemical structure of ZCP-6S were characterized by laser particle size analyzer,transmission electron microscopy(TEM)and Fourier transform infrared(FTIR)spectroscopy.The results indicated that the nanoparticles were spherical in shape,with small particle size and uniform distribution.6S might interact with zein through non-covalent bonds.The in vitro simulated digestion results showed that ZCP-6S increased the bioaccessibility of 6S to(75.34±9.82)％.Moreover,ZCP-6S significantly enhanced the uptake and transport of 6S by Caco-2 cells.After 4 h treatment,the cell uptake increased by(0.36±0.06)pg/mg.The concentration of 6S in the basolateral side of the Caco-2 cell model increased by(1.06±0.06)μg/mL.In addition,pharmacokinetic studies showed that the relative oral bioavailability of 6S increased by 3.28 times after nanoparticle encapsulation.In summary,the bioaccessibility,cellular absorption and oral bioavailability of 6S were effectively improved by its encapsulation into zein nanoparticles.关键词
6-姜烯酚/玉米醇溶蛋白/酪蛋白酸钠/纳米颗粒/生物利用度Key words
6-shogaol/zein/sodium caseinate/nanoparticles/bioavailability分类
轻工业引用本文复制引用
高就,吴俊杰,焦文雅,陈宇洋,祝文轩,桑亚新,王向红..6-姜烯酚玉米醇溶蛋白纳米颗粒的构建及其生物利用度分析[J].食品科学,2024,45(9):44-50,7.基金项目
河北省重点研发计划项目(21327314D) （21327314D）
河北省三期蔬菜产业技术体系创新团队项目(HBCT2023100206) （HBCT2023100206）
