食品科学2024，Vol.45Issue(9)：102-108,7.DOI:10.7506/spkx1002-6630-20230710-109
马乳酒样乳杆菌马乳酒样亚种real-time PCR检测方法的建立与应用
Establishment and Application of Real-Time PCR Detection Method for Lactobacillus kefiranofaciens subsp.kefiranofaciens
吕厚姣 1李欣媛 1白小佳 1贾龙刚 1耿伟涛 1王艳萍1
作者信息
- 1. 天津科技大学食品科学与工程学院,天津 300457
- 折叠
摘要
Abstract
This study developed a real-time polymerase chain reaction(real-time PCR)method for the detection of Lactobacillus kefiranofaciens subsp.kefiranofaciens.Based on the 16S rDNA gene sequence and whole genome sequence of the type strain of L.kefiranofaciens subsp.kefiranofaciens ZW3,specific primers were designed and screened.The fluorescent dye SYBR Green I was used in the real-time PCR method.Its specificity,sensitivity and repeatability were evaluated,and this method was applied to detect several strains of this species and its mixtures with other lactic acid bacteria.The results showed that the proposed method was highly specific,sensitive and repeatable.The standard curve was linear with a determination coefficient(R2)of 0.965.Moreover,this method could specifically detect L.kefiranofaciens subsp.kefiranofaciens from its mixture with other lactic acid bacteria.In summary,the real-time PCR method could quickly and accurately detect L.kefiranofaciens subsp.kefiranofaciens,providing a new method for the specific qualitative and quantitative detection of L.kefiranofaciens subsp.kefiranofaciens.关键词
马乳酒样乳杆菌马乳酒样亚种/实时聚合酶链式反应/特异性引物Key words
Lactobacillus kefiranofaciens subsp.kefiranofaciens/real-time polymerase chain reaction/specific primers分类
生物科学引用本文复制引用
吕厚姣,李欣媛,白小佳,贾龙刚,耿伟涛,王艳萍..马乳酒样乳杆菌马乳酒样亚种real-time PCR检测方法的建立与应用[J].食品科学,2024,45(9):102-108,7.
