食品科学2024，Vol.45Issue(9)：116-123,8.DOI:10.7506/spkx1002-6630-20230601-005
神经酸对H2O2诱导PC12细胞氧化损伤的保护作用
Protective Effect of Nervonic Acid on Oxidative Damage of PC12 Cells Induced by H2O2
摘要
Abstract
This study developed a cell model of oxidative damage by treating PC 12 cells for 24 h with 200 μmol/L H2O2 and determined the degree of oxidative stress by assaying the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and lactate dehydrogenase(LDH)and the level of malondialdehyde(MDA).Moreover,cell apoptosis and reactive oxygen species(ROS)levels were evaluated.Western blot and real-time polymerase chain reaction(PCR)were used to detect the protein and mRNA expression levels of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),caspase-3,nuclear factor E2 related factor 2(Nrf2),kelch-like ECH-associated protein 1(Keap1),and heme oxygenase-1(HO-1).The results showed that after being treated with 200 μmol/L H2O2 for 24 h,the survival rate of PC12 cells was 60.12％.Cytotoxicity experiments showed that nervonic acid could significantly reduce the contents of LDH and MDA,inhibit excessive production of ROS,and enhance the activities of SOD and GSH-Px in H2O2-injuried cells.In addition,it significantly upregulated the expression of Bcl-2,Nrf2 and HO-1,and downregulated the expression of caspase-3,Bax,and Keap1.In summary,nervonic acid has a protective effect on H2O2-induced oxidative damage in PC12 cells by a mechanism associated with the activation of the Nrf2/HO-1 signaling pathway.关键词
神经酸/氧化损伤/PC12细胞/信号通路Key words
nervonic acid/oxidative damage/PC12 cells/signaling pathway分类
生物科学引用本文复制引用
玛依乐·艾海提,刘垚杰,李建科..神经酸对H2O2诱导PC12细胞氧化损伤的保护作用[J].食品科学,2024,45(9):116-123,8.基金项目
"十三五"国家重点研发计划重点专项(2019YFD1002400 （）
2019YFD1002403) （）
陕西省科技计划项目(2020QFY08-03) （2020QFY08-03）
