食品科学2024，Vol.45Issue(9)：173-180,8.DOI:10.7506/spkx1002-6630-20230512-111
PI3K/AKT信号通路对冷却滩羊肉贮藏期间细胞凋亡的影响
Effect of Phosphoinositide 3-Kinase/Protein Kinase B Signaling Pathway on Cell Apoptosis during Chilled Storage of Tan Sheep Meat
摘要
Abstract
The hindleg meat of Tan sheep was injected postmortem with the phosphoinositide 3-kinase(PI3K)inhibitor LY294002 at a concentration of 10 μmol/L and stored at 4 ℃.After 0,2,4,6 and 8 days,the protein expression of PI3K and protein kinase B(AKT)was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot so as to verify whether the PI3K/AKT signaling pathway was effectively inhibited.Meanwhile,we analyzed changes in energy factors,oxidative stress levels,the degree of mitochondrial damage and cysteine aspastic acid-specific protease 3(caspase-3)activity in order to explore the mechanism by which the PI3K/AKT signaling pathway induces the pathways of cell apoptosis during chilled storage of Tan sheep meat.The results showed that during storage,the protein expression levels of PI3K and AKT showed a decreasing trend,indicating that LY294002 inactivated the PI3K/AKT signaling pathway.Compared with the control group,LY294002 treatment significantly reduced mitochondrial ATP,glycogen content,succinate dehydrogenase(SDH)and citrate synthase(CS)activity(P＜0.05),gradually increased mitochondrial reactive oxygen species(ROS)levels,elevated the levels of oxidative stress and the opening of mitochondrial permeability transition pore(MPTP),significantly decreased membrane potential(P＜0.05),and significantly augmented the activity of caspase-3(P＜0.05),indicating that the PI3K/AKT signaling pathway mediates the activation of downstream caspase-3 by promoting the production of ROS to interact with PI3K,damaging the structure and function of mitochondria,leading to the opening of MPTP and decreased membrane potential,and ultimately inducing cell apoptosis.关键词
磷脂酰肌醇3激酶/蛋白激酶B通路/冷却滩羊肉/细胞凋亡Key words
phosphoinositide 3-kinase/protein kinase B pathway/chilled Tan sheep meat/cell apoptosis分类
轻工纺织引用本文复制引用
张倩,罗瑞明,陈雪妍,李荣,王金霞,胡丽筠..PI3K/AKT信号通路对冷却滩羊肉贮藏期间细胞凋亡的影响[J].食品科学,2024,45(9):173-180,8.基金项目
国家自然科学基金地区科学基金项目(31860427) （31860427）
