中国食品添加剂2024，Vol.35Issue(4)：201-207,7.DOI:10.19804/j.issn1006-2513.2024.4.024
积雪草酸通过调控TLR4/NF-κB/NLRP3信号通路改善非酒精性脂肪肝的机制研究
Mechanism of asiatic acid improving nonalcoholic fatty liver disease by regulating TLR4/NF-κB/NLRP3 signaling pathway
摘要
Abstract
To investigate the effect and mechanism of the natural active ingredient asiatic acid(AA)on nonalcoholic fatty liver disease(NAFLD)induced by high-fat diet.Forty male C57BL/6J mice were divided into normal group,model group,AA low dose group and AA high dose group.After 4 weeks of high-fat diet,AA intervention was given for 12 weeks.After the experiment,the weight of mice livers and the mice liver index were measured.The content of biochemical indicators related to mice fatty liver in mice serum were measured by Spectrophotometer.The expression level of related inflammatory factors in mice serum were detected by enzyme linked immunosorbent assay.Proteins related to TLR4/NF-κB/NLRP3 signaling pathway in mice liver were detected by Western blotting.The pathological changes of liver in mice were observed by various staining methods.The results showed that AA could effectively improve the NAFLD mice general indicators,biochemical indicators,inflammatory factors,and proteins related to TLR4/NF-κB/NLRP3 signaling pathway.AA could inhibit liver inflammation and ameliorate NAFLD by downregulating TLR4/NF-κB/NLRP3 signaling pathway.关键词
积雪草酸/非酒精性脂肪肝/TLR4/NF-κB/NLRP3/信号通路Key words
asiatic acid/non-alcoholic fatty liver disease/TLR4/NF-κB/NLRP3/signal pathway分类
轻工纺织引用本文复制引用
叶子昊,祝可欣,贺宇涵,陈灵,张景正..积雪草酸通过调控TLR4/NF-κB/NLRP3信号通路改善非酒精性脂肪肝的机制研究[J].中国食品添加剂,2024,35(4):201-207,7.基金项目
江苏卫生健康职业学院院级重点课题(JKB2021005) （JKB2021005）
江苏省大学生创新创业训练计划项目(202014255002Y) （202014255002Y）
江苏卫生健康职业学院院级重点课题(JKB2021005). （JKB2021005）
