中国农业科技导报2024，Vol.26Issue(5)：30-43,14.DOI:10.13304/j.nykjdb.2024.0186
玉米木质素合成途径基因ZmCCoAOMT1功能研究及转录组分析
Functional Study and Transcriptome Analysis of Corn Gene ZmCCoAOMT1 Involved in Lignin Synthesis Pathway
摘要
Abstract
Silage corn with high-quality roughage source for animal husbandry is an excellent forage crop.Caffeoyl-CoA-O-methyltransferase(CCoAOMT),a key methyltransferase,plays an important role in the lignin synthesis.In this study,the EMS single-base mutant of ZmCCoAOMT1 gene was obtained from EMS mutant library of corn inbred line B73,and the function of ZmCCoAOMT1 was demonstrated based on phenotypic analysis,physiological and biochemical analysis and gene expression analysis.It was found that the mutation of ZmCCoAOMT1 reduced the degree of lignification,significantly decreased the stem lignin content,and declined G(guaiacyl lignin)and S(syringa lignin)monomers contents,whereas increased in vitro digestibility of dry matter.The expression of ZmCCoAOMT1 in the stalks of the mutant was significantly reduced compared to the wild type.Transcriptome analysis analysis showed that the mutation of ZmCCoAOMT1 gene might change the expression of related genes involved in the phenylpropanoid metabolic pathway,leading to variation in lignin content and monomer composition.Therefore,ZmCCoAOMT1 gene would be valuable in corn breeding for high-digestibility and high-quality silage,and above results provided an important genetic basis for elucidating the mechanism of lignin anabolic pathway.关键词
青贮玉米/木质素/转录组分析/ZmCCoAOMT1Key words
silage corn/lignin/transcriptome analysis/ZmCCoAOMT1分类
农业科技引用本文复制引用
徐佳睿,王逸茹,赵绍赓,李坤,郑军..玉米木质素合成途径基因ZmCCoAOMT1功能研究及转录组分析[J].中国农业科技导报,2024,26(5):30-43,14.基金项目
国家重点研发计划项目(2023YFD1200500). （2023YFD1200500）
