植物营养与肥料学报2024，Vol.30Issue(3)：505-514,10.DOI:10.11674/zwyf.2023436
缺磷限制带状复合种植大豆光合作用对光照诱导的响应
Phosphorus deficiency limits photosynthetic induction response to light in soybeans under strip compound planting systems
摘要
Abstract
[Objective]When intercropping with maize(Zea mays L.),the low-tier crop soybean[Glycine max(L.)Merr.]would suffer yield limitations due to fluctuating light conditions.As phosphorous(P)involves in various physiological processes,we studied the effect of P nutrition on photosynthesis during light induction,trying to enhance soybean photosynthetic efficiency and production potential through P nutrient management.[Method]Field experiments were conducted in Sichuan Agriculture University,the cropping system was maize and soybean intercropping.P fertilizer application(CK)and no application(-P)on soybean was set up as the treatments.At branching stage of soybean,leaf gas exchange parameters,P content,and the relevant physiological parameters were measured under both steady-state light and light-induced processes.The parameters included net photosynthesis rate(A),stomatal conductance(gs),intercellular CO2 concentration(Ci),transpiration rate(Tr),and electron transfer rate(J),as well as P content and photosynthetic pigment levels.Additionally,proportions of stomatal and biochemical photosynthetic limitation factors were calculated.[Results]Compared with CK,-P significantly inhibited the growth of soybean,the grain yield and plant dry weight at maturing stage were reduced by 24.5%and 23.9%,respectively.-P significantly reduced the total and inorganic P contents of leaves at the branching stage,but did not affect the contents of chlorophyll a,chlorophyll b,and carotenoids.Under steady-state saturated light,-P decreased the leaf A and Tr by 30.9%and 39.5%,but did not impact the gs significantly,resulting in a significant increase in Ci.-P decreased the stomatal limit value of soybean leaves by 16.7%,implying that non-stomatal factors were the dominant factor causing the decrease in A under steady-state saturated illumination.During the induction period of transitioning from low to high light,the induction rates of A and gs in CK were faster than those in-P treatment,while the induction rate of J showed no significant difference between the two treatments.Photosynthetic limitation analysis revealed no significant difference in cumulative stomatal limitation and biochemical limitation during light induction under CK treatment.However,cumulative stomatal limitation reached 65.7%under-P condition,significantly higher than biochemical limitation.It showed that stomata was the main factor limiting its photosynthetic induction rate under phosphorus deficiency conditions.[Conclusions]Phosphorus deficiency mainly limits the response of stomatal conductance of soybean leaves to light induction under strip compound planting,resulting in a decrease in photosynthetic efficiency and yield.Therefore,reasonable phosphorus fertilizer application is of great significance to improve the photosynthetic efficiency of soybeans in soybean-maize strip compound planting under fluctuating light environment and reduce yield loss.关键词
大豆/光合作用/光照诱导/光合限制/缺磷胁迫Key words
soybean/photosynthesis/light induction/photosynthetic limitation/phosphorus deficiency引用本文复制引用
李林宽,张锡洲,陈静,叶代桦,刘涛..缺磷限制带状复合种植大豆光合作用对光照诱导的响应[J].植物营养与肥料学报,2024,30(3):505-514,10.基金项目
四川省自然科学基金面上项目(2023NSFSC0225) （2023NSFSC0225）
四川省自然科学基金青年基金项目(2022NSFSC1664) （2022NSFSC1664）
"天府峨眉计划"青年人才项目. （）
