中国食品添加剂2024，Vol.35Issue(4)：81-90,10.DOI:10.19804/j.issn1006-2513.2024.4.010
响应面法优化屎肠球菌T2-2产γ-氨基丁酸的发酵条件
Optimization of fermentation conditions for γ-aminobutyric acid production by Enterobacterium faecium T2-2 using response surface methodology
摘要
Abstract
Enterococcus faecium T2-2 was used as the research object,which was isolated from traditional pickles.The fermentation conditions were optimized by single factor experiment and response surface method to increase GABA yield.The results showed that the optimum fermentation conditions for GABA production by Enterococcus faecium T2-2 were as follows:temperature 30℃,Enterococcus faecium T2-2 inoculation amount 7.50%,L-glutamate sodium addition 18.93 g/L,and pH 6.0,time 1 d,the bacterial growth rate was 0.338,the enzyme activity was 3.177 μg/mL,and under these conditions,the GABA yield was 1.190 mg/mL,which was 63.01%higher than that before optimization.This study provides a theoretical basis for improving the GABA content produced by lactic acid bacteria.关键词
屎肠球菌T2-2/γ-氨基丁酸/谷氨酸脱羧酶/响应面分析法/优化Key words
Enterobacterium faecium T2-2/γ-aminobutyric acid/glutamate decarboxylase/response surface analysis/optimization分类
轻工纺织引用本文复制引用
张桂英,陈晖,孙卫宁,杜文娟,姜龙波,桑大席,朱静..响应面法优化屎肠球菌T2-2产γ-氨基丁酸的发酵条件[J].中国食品添加剂,2024,35(4):81-90,10.基金项目
山西省基础研究计划青年科学研究项目(自由探索类)(202203021212463) （自由探索类）
信阳农林学院高水平科研孵化器建设基金项目(FCL202014) （FCL202014）
2022信阳农林学院科技创新团队(XNKJTD-001) （XNKJTD-001）
河南省2022年高校研究性教学改革研究项目(2022SYJXLX119). （2022SYJXLX119）
