现代食品科技2024，Vol.40Issue(4)：8-17,10.DOI:10.13982/j.mfst.1673-9078.2024.4.0510
葛根素缓解非酒精性脂肪肝小鼠肝脏代谢紊乱
Puerarin Relieves Liver Metabolic Disorders in Mice with Nonalcoholic Fatty Liver Disease
摘要
Abstract
High fat and/or high fructose intakes lead to excessive fat storage in the liver,resulting in nonalcoholic fatty liver disease(NAFLD),whose global incidence rate is 25.2%and rising rapidly.In this study,the effects of puerarin intervention on NAFLD were investigated in a mouse model induced by high-fructose and high-fat diets.The results show that the body weight and liver index of NAFLD mice decreased by 13.7%and 14.9%,respectively;the H&E staining indicated that the liver tissue damage was mitigated and the fat vacuoles almost disappeared;and the concentrations of inflammatory factors TNF-α,IL-1β,and IL-6 decreased by 62.9%,60.5%and 61.0%,respectively,as determined by ELISA.LC-MS non-targeted metabolomic analysis of liver metabolism in the NAFLD mice showed that puerarin increased the levels of metabolites L-methionine,L-serine,L-asparagine,L-phenylalanine,and aminoadipic acid;modulated the disturbances of cysteine and methionine metabolism pathways,glycine,serine,and threonine metabolism pathways,and phenylalanine,tyrosine and tryptophan biosynthesis pathways.In addition,puerarin down-regulated the levels of the lipid metabolite oleic acid and promoted the degradation of sphingolipids and regulated the levels of pentose phosphate metabolites,vitamin metabolites,and purine and pyrimidine metabolites.In summary,puerarin regulates the disturbances of amino acid and lipid metabolism pathways,and plays a protective role against NAFLD induced by high-fructose and high-fat diets,laying an experimental foundation for dietary regulation of fat metabolism and even fatty liver.关键词
葛根素/非酒精性脂肪肝/非靶向代谢组学/代谢物Key words
puerarin/nonalcoholic fatty liver/non-targeted metabonomics/metabolites引用本文复制引用
丁文姣,葛晶晶,吴优,虞雨娟,周峰,阮征..葛根素缓解非酒精性脂肪肝小鼠肝脏代谢紊乱[J].现代食品科技,2024,40(4):8-17,10.基金项目
国家自然科学基金项目(32060539) （32060539）
