灰树花多肽-锌螯合物对孕期缺锌鼠后代的改善作用OA北大核心CSTPCD

Grifola frondosa polypeptide-zinc chelate(GPs-Zn)is the chelation product of Grifola frondosa polypeptides(GPs)and zinc ions.GPs-Zn was subjected to structural characterization using scanning electron microscopy,X-ray powder diffraction,infrared spectroscopy,and ultraviolet spectroscopy.The effects of GPs-Zn on the offspring of zinc-deficient pregnant mice were explored by constructing an adult female ICR mouse model of zinc deficiency.Characterization results showed that GPs and zinc ions formed a novel substance through chelation;IR spectral comparison revealed that both amino and carboxyl groups on the peptide chain participated in the coordination reaction of zinc ions.The UV spectrum of GPs-Zn exhibited a red shift attributed to the binding of the carbonyl atoms of the peptides to zinc ions,indicating that GPs bound to zinc ions to form GPs-Zn.Animal experiments showed that GPs-Zn increased the thymus index of zinc-deficient offspring by 78.69%(female rats)and 87.55%(male rats),the spleen index by 40.28%(female rats)and 43.22%(male rats),the body weight by 89.98%(female rats)and 88.30%(male rats),the superoxide dismutase(SOD)activity in serum by 108.07%(female rats)and 26.16%(male rats),and the zinc concentration by 14.74%(female rats)and 29.33%(male rats),but decreased the serum alkaline phosphatase(AKP)activity by 52.28%(female rats)and 62.48%(male rats).GPs-Zn can improve the thymus index,spleen index,SOD activity,zinc concentration,and AKP activity of the offspring of zinc-deficient pregnant mice.