现代食品科技2024，Vol.40Issue(4)：73-83,11.DOI:10.13982/j.mfst.1673-9078.2024.4.0377
罗伊氏粘液乳杆菌K07的生物学特性及其基因分析
Biological Characteristics of Limosilactobacillus reuteri K07 and Its Genetic Analysis
摘要
Abstract
Two strains of Limosilactobacillus reuteri(K07,K08)were isolated from traditional fermented cheese of Zhaosu County,Xinjiang Province.The growth,acid production,acid tolerance,bile salt tolerance,tolerance to simulated gastrointestinaltract,reuterin-producing capacity and antioxidant activity of the two strains were evaluated.In addition,the bacteriostatic performance of two strains was determined and genetic analysis of the K07 strain was performed.The results showed that both strains entered the late logarithmic growth phase after 12 hours of culture,and the endpoint pH of fermentation was 4.45.K07 exhibited stronger tolerance to acid,bile salt and the simulated gastrointestinal tract as well as higher antioxidant ability than K08.The survival rate of K07 was 73.27%after the treatment at pH 2.50 for 4 h.For the K07 strain,it took 5.50 hours for the OD600 nm to increase by 0.3 units under the stress of 0.15%bile salt.The survival rate of K07 in the simulated gastrointestinal test was 74.13%.The antioxidant activities of the cell-free extracts of the two strains were higher than those of the intact cells,with the scavenging rates of the cell free extracts of K07 for DPPH free radicals and hydroxyl free radicals were 42.39%and 15.69%,respectively.with the reducing power being17.96%.The amount of reuterin produced by K07 was 183.05 mmol/L,which was significantly higher than that of K08.Also,K07 exhibited better antimicrobial performance.The diameters of inhibition zones for Candida albicans and Streptococcus agalactiae were 22.20 mm and 18.26 mm,respectively.The full length of K07 genome was 1 950 440 bp,with 2067 predicted protein coding genes.The content of GC was 38.69%.The research results showed that Limosilactobacillus reuteri K07 had good probiotic properties,which provides a theoretical basis for subsequent industrial applications.关键词
罗伊氏粘液乳杆菌/益生特性/基因分析Key words
Limosilactobacillus reuteri/prebiotic properties/genetic analysis引用本文复制引用
黄志钰,黎旭,李幸,金庭飞,陶纯长,刘宗争..罗伊氏粘液乳杆菌K07的生物学特性及其基因分析[J].现代食品科技,2024,40(4):73-83,11.基金项目
新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划及国际科技合作计划)项目(2022E01028) （上海合作组织科技伙伴计划及国际科技合作计划）
