现代食品科技2024，Vol.40Issue(4)：104-112,9.DOI:10.13982/j.mfst.1673-9078.2024.4.0427
酶解糖化滇黄精多糖的结构表征及其免疫活性
Enzymatic Saccharification of Polygonatum kingianum Coll.et Hemsl Polysaccharides:Structural Characterization and Immunological Activity
摘要
Abstract
The structural characteristics and immunomodulatory activity of Polygonatum kingianum Coll.et Hemsl polysaccharides were investigated under conditions of enzymatic saccharification.The results revealed that among the products of enzymatic saccharification in P.kingianum(SPKP),the contents of polysaccharides and reducing sugars accounted for 94.05%and 4.1%,respectively.Structural analysis of the polysaccharides using Fourier transform infrared spectroscopy revealed that these had furanose-specific absorption peaks at 1 016～1 147 and 931 cm-1,whereas the monosaccharide composition and molar ratios were arabinose:galactose:glucose:mannose:fructose(0.032:0.117:0.419:0.048:0.383),and the molecular weight(Mw)was 3 820 u.The main type of linkage was →4-Glcp-1→(47.1%),and the corresponding branching degree was 34.1%.Combined with the results of the iodine-potassium iodide and Congo Red staining experiments,SPKP were inferred to be a three-stranded helical polysaccharides containing complex branches.The findings of RAW264.7 cell experiments indicated that P.kingianum polysaccharides are non-toxic within a wide range of concentrations and can promote cell proliferation,phagocytic capacity,and production of NO.Accordingly,it can be speculated that SPKP have good immunomodulatory activities,which are associated with polysaccharide structures.Our findings in this study may provide a reference for determining the structure-function relationships between polysaccharides and immunomodulatory activity,as well as the development of new methods for processing P.kingianum.关键词
滇黄精/酶解糖化/多糖结构/免疫活性Key words
Polygonatum kingianum Coll.et Hemsl/enzymatic saccharification/polysaccharide structure/immunological activity引用本文复制引用
黄俊源,袁晚晴,苏艺,杨淑婷,黎攀,陈建萍,杜冰..酶解糖化滇黄精多糖的结构表征及其免疫活性[J].现代食品科技,2024,40(4):104-112,9.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-21) （CARS-21）
