现代食品科技2024，Vol.40Issue(4)：225-235,11.DOI:10.13982/j.mfst.1673-9078.2024.4.0406
亚临界萃取琥珀精油工艺优化及活性分析
Optimizing the Subcritical Extraction Process of Amber Essential Oil and Analysis of Its Functional Activity
摘要
Abstract
Subcritical fluid extraction of amber essential oil was conducted using amber as the raw material.The extraction process was optimized using extraction yield as the metric.The extracted amber essential oil was subjected to GC-MS analysis to determine the major chemical components and their relative contents,and also subjected to in vitro antioxidant analysis and tyrosinase inhibition tests.The results show that the optimal process conditions were a liquid-to-material ratio of 2.1:1(mL:g),an extraction temperature of 58℃,and an extraction duration of 51 min,which led to an extraction yield of 1.61%.The GC-MS analysis revealed that 40 peaks were isolated from the amber essential oil,with 30 compounds being identified.Among them,monoterpenes were the most abundant compounds,mainly comprising cymene(29.39%),(-)-borneol(18.02%),fenchol(8.46%),(-)-camphor(7.12%),and eucalyptol(6.40%),which are the main contributors to the unique aroma of amber essential oil.The amber essential oil showed strong antioxidant activity,with IC50 values of 12.66 mg/mL and 2.91 mg/mL for the scavenging of DPPH and ABTS+ radicals,respectively.The inhibition of tyrosinase by amber essential oil is non-competitive,with an IC50 value of 1.13 mg/mL,and its whitening activity is higher than that of arbutin at the same concentration.关键词
琥珀精油/亚临界流体萃取/响应面/气相色谱-质谱联用/抗氧化活性/美白活性Key words
amber essential oil/subcritical fluid extraction/response surface/GC-MS/antioxidant activity/whitening activity引用本文复制引用
杨媛媛,唐语谦,杨继国..亚临界萃取琥珀精油工艺优化及活性分析[J].现代食品科技,2024,40(4):225-235,11.基金项目
东莞市科技特派员项目(20211800500422) （20211800500422）
岭南现代农业科学与技术广东省实验室河源分中心自主科研项目(DT20220027) （DT20220027）
