现代食品科技2024，Vol.40Issue(4)：341-349,9.DOI:10.13982/j.mfst.1673-9078.2024.4.0517
萝卜硫苷的高效纯化技术研究进展
Research Progress on the Efficient Purification of Glucoraphanin
摘要
Abstract
Glucoraphanin(RAA)is a glucosinolate widely found in cruciferous plants,the metabolites of which have been established to have anti-cancer effects.However,the purity of RAA extracted from cruciferous plants is low,which limits its effective utilization,and consequently,it is necessary to develop an effective process for RAA purification.Although the main technique currently used for the purification of RAA is chromatographic purification,this procedure has notable drawbacks,including low recovery rates and secondary contamination.As an alternative procedure,membrane purification,which is the most commonly used method for the separation and purification of glycosides,requires only simple equipment and entails simple operation.Moreover,it is less prone to secondary contamination.Accordingly,this separation procedure is considered a potential technique for the purification of RAA.It is speculated that combined chromatographic and membrane purification could facilitate the effective recovery of RAA and enhance the purity of the recovered product,thereby overcoming the problems of low recovery and secondary contamination of RAA associated with the sole use of chromatographic purification.This study focuses mainly on the main methods currently used for the purification of RAA and assesses the feasibility of the combined application of chromatography and membrane purification techniques for RAA purification,thereby providing a methodological reference for the efficient purification of RAA.关键词
萝卜硫苷/十字花科植物/高效纯化/色谱纯化技术/膜纯化技术Key words
glucoraphanin/cruciferous plant/efficient purification/chromatographic purification technology/membrane purification technology引用本文复制引用
李金旺,甄少波,李修德,周麟依,刘奕忍,李金峰..萝卜硫苷的高效纯化技术研究进展[J].现代食品科技,2024,40(4):341-349,9.基金项目
国家自然科学基金青年基金项目(32302104) （32302104）
中国劳动关系学院校级教改项目(JG23016) （JG23016）
