香蕉HOS15基因的鉴定及枯萎病菌胁迫下的表达分析OACSTPCD

In order to study whether HOS15(HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15)is involved in the regulation of banana resistance process,the Arabidopsis HOS15 protein se-quence is used to identify banana HOS15(MaHOS15)gene using various bioinformatics analysis tools,and u-sing the highly resistant variety'Nantianhuang'and the susceptible variety'Williams'as experimental objects,the expression analysis of MaHOS15 under the stress of fusarium wilt and its potential mechanism of regula-ting plant diseases are studied.The results show that the LisH domain and WD40 repeat protein sequence in MaHOS15 protein are highly conserved.The secondary structure of MaHOS15 protein has α-helix,β-fold,ex-tended chain and random coil structure,and the random coil is the main structure accounting for 46.2％.Sub-cellular localization is in the nucleus,which can be supposed thatMaHOS15 may be involved in the regulation of banana fusarium wilt in the nucleus.MaHOS15 may respond to SA signaling pathway,MeJA signaling pathway and abscisic acid reaction.It is speculated that MaHOS15 is a key gene in the negative regulation of plant defense against pathogens in the process of plant disease resistance.