乳业科学与技术2024，Vol.47Issue(1)：1-7,7.DOI:10.7506/rykxyjs1671-5187-20240207-007
不同泌乳期山羊乳与牛乳中类固醇激素含量差异
Differences in Steroid Hormone Contents between Goat and Cow Milk at Different Lactation Stages
摘要
Abstract
Purpose:To understand the differences in steroid hormone contents between goat and cow milk at different lactation stages.Methods:The contents of steroid hormones in goat and cow milk at different lactation stages were investigated using ultra-high liquid chromatography-mass spectrometry(UPLC-MS).Gradient elution was performed using 0.5 mmol/L ammonium fluoride aqueous solution as mobile phase A and methanol as mobile phase B at a flow rate of 0.3 mL/min,and the target analytes were qualitatively and quantitatively detected using an electrospray ionization(ESI)source in the multiple reactions monitoring(MRM)mode.Results:A total of 11 steroid hormones,17α-hydroxyprogesterone,17α-pregnenolone,epi-testosterone,pregnenolone,progesterone,androstenedione,testosterone,estradiol,estrogen,cortisol,and cortisone,were detected from goat and cow milk samples.At the early lactation stage,the contents of 17α-hydroxyprogesterone,estrogen and cortisol showed significant differences between goat and cow milk(P<0.05),and the contents of progesterone and progesterone showed extremely significant differences(P<0.01);at the full lactation stage,significant differences in the contents 17α-hydroxyprogesterone,17α-pregnenolone,androstenedione,and cortisone(P<0.05),and extremely significant differences in the contents of progesterone and pregnenolone were found(P<0.01);at the late lactation stage,significant differences in the contents of 17α-hydroxyprogesterone,androstenedione,estrogen,and cortisol(P<0.05)were observed as well as extremely significant differences in the content of 17α-hydroxypregnenolone,pregnenolone,and progesterone(P<0.01).Conclusion:The differences in steroid hormone contents between goat and cow milk may be due to different pregnancy status.关键词
超高效液相色谱-串联质谱/山羊乳/牛乳/泌乳期/类固醇激素/含量差异Key words
ultra-high liquid chromatography-tandem mass spectrometry/goat milk/cow milk/lactation period/steroid hormones/content differences分类
轻工纺织引用本文复制引用
沙日阿康,任新阳,屈颖新,王广,余梦琦,陈璐,袁宇欣,李广..不同泌乳期山羊乳与牛乳中类固醇激素含量差异[J].乳业科学与技术,2024,47(1):1-7,7.基金项目
陕西省农业协同创新推广联盟项目(LMZD202002) （LMZD202002）
陕西省农业科技创新驱动项目(NYKJ-2021-ST-03) （NYKJ-2021-ST-03）
陕西省技术创新引导专项(2022QFY11-01) （2022QFY11-01）
