乳业科学与技术2024，Vol.47Issue(1)：8-13,6.DOI:10.7506/rykxyjs1671-5187-20240207-006
β-乳球蛋白与二十二碳六烯酸互作效应对β-乳球蛋白致敏性机制的影响
Impact of Interaction with Docosahexaenoic Acid on the Allergenicity of β-Lactoglobulin
摘要
Abstract
β-Lactoglobulin(β-LG)is a major allergen in cow's milk.In this study,synchronous fluorescence spectroscopy,three-dimensional fluorescence spectroscopy,circular dichroism(CD)spectroscopy,molecular docking,and indirect competitive enzyme-linked immunosorbent assay were used to explore the interaction of docosahexaenoic acid(DHA)with β-LG and its influence on the allergenicity of β-LG.The results showed that the binding of DHA to β-LG significantly changed the microenvironment of the aromatic residues of β-LG and increased its polarity.DHA bound to β-LG to form a stable complex through hydrophobic interactions,changing the secondary structure of β-LG and resulting in folding of its backbone.The relative content of β-sheet increased and then decreased with increasing concentration of DHA,while the relative content of α-helix showed an opposite trend.However,the relative contents of β-turn and random coil showed irregular changes.In addition,the antigenicity of β-LG decreased after combination with DHA,and the immunoglobulin E(IgE)binding ability inhibition rate was about 29%by 3 mmol/L DHA.关键词
β-乳球蛋白/二十二碳六烯酸/互作效应/致敏性Key words
β-lactoglobulin/docosahexaenoic acid/interaction effect/allergenicity分类
轻工纺织引用本文复制引用
周韦妮,梁肖娜,王婧,吴婷,沃俞馨,杜雅宣,姬雅雅,沙祎铖,陈代昌,赵倩..β-乳球蛋白与二十二碳六烯酸互作效应对β-乳球蛋白致敏性机制的影响[J].乳业科学与技术,2024,47(1):8-13,6.基金项目
大学生创新训练计划项目(202310323111Y) （202310323111Y）
江苏省高等学校基础科学(自然科学)研究项目(23KJD55001) （自然科学）
淮安市基础研究计划(联合专项)项目(HABL2023001) （联合专项）
