乳业科学与技术2024，Vol.47Issue(1)：14-18,5.DOI:10.7506/rykxyjs1671-5187-20240401-014
离子色谱法测定乳制品中的亚硝酸盐和硝酸盐
Determination of Nitrite and Nitrate in Milk Products by Ion Chromatography
摘要
Abstract
An ion chromatography with conductivity detector(IC-CD)method was developed for the determination of nitrite and nitrate in milk products.Samples were dissolved in water and added with potassium ferrocyanide and zinc sulphate solutions for protein precipitation.The supernatant was cleaned up on C18,Ag,and Na solid-phase extraction(SPE)columns,and analyzed by IC-CD.An external standard method was used for quantification.The IC-CD method was validated.Good linearity between the concentrations of nitrite(NaNO2)and nitrate(NaNO3)in the range of 0.01-0.20 mg/L and 0.1-2.0 mg/L,respectively and peak area was observed with correlation coefficient equal to or higher than 0.99.The limits of quantitation for nitrite and nitrate were 0.2 and 0.4 mg/kg,respectively.The spiked recoveries ranged from 92.5%to 102.0%,and the relative standard deviation(RSD)for precision from 1.5%to 6.6%.This method is accurate,repeatable,simple and applicable to the determination of nitrite and nitrate in milk products.关键词
乳制品/亚硝酸盐/硝酸盐/离子色谱Key words
milk products/nitrite/nitrate/ion chromatography分类
轻工纺织引用本文复制引用
印杰,郑国建,叶寅颖..离子色谱法测定乳制品中的亚硝酸盐和硝酸盐[J].乳业科学与技术,2024,47(1):14-18,5.基金项目
上海市科学技术委员会科研计划项目(22DZ2202400) （22DZ2202400）
