中国免疫学杂志2024，Vol.40Issue(4)：693-697,5.DOI:10.3969/j.issn.1000-484X.2024.04.004
SARS-CoV-2广谱中和抗体性质鉴定
Identification of broadly neutralizing antibody against SARS-CoV-2
摘要
Abstract
Objective:To identify XMA09,a broadly neutralizing antibody against severe acute respiratory syndrome coronavi-rus 2(SARS-CoV-2),and to explore the potential mechanism of XMA09 to broadly neutralize variants of SARS-CoV-2,so as to pro-vide a reference for vaccine design and broadly neutralizing antibody screening against SARS-CoV-2.Methods:XMA09 protein was expressed and purified by ExpiCHO expression system and Protein A chromatography column.The key amino acid sites on receptor binding domain(RBD)recognized by XMA09 were identified by Cryo-EM,and the affinity of XMA09 to Spike protein of SARS-CoV-2 and its variants was detected by indirect ELISA and Surface Plasmon Resonance(SPR).The pseudovirus neutralization assay based on vesicular stomatitis virus(VSV)was used to detect the neutralization ability of XMA09 to wild-type and variants of SARS-CoV-2.Results:In this study,it was found that the epitopes recognized by XMA09 were conservative,who has an excellent binding ability to Spike protein of many kinds of variants,and could neutralize the variants of concern(VOCs),including the widespread BA.4/5.Conclusion:XMA09 is a broadly neutralizing antibody,which has the potential to be used as a therapeutic antibody against SARS-CoV-2,and can provide reference for broad-spectrum vaccine design and antibody drug development against SARS-CoV-2.关键词
SARS-CoV-2/受体结合结构域(RBD)/广谱中和抗体Key words
SARS-CoV-2/Receptor binding domain(RBD)/Broadly neutralizing antibody分类
医药卫生引用本文复制引用
陈秀婷,汪毅祯,商慧娴,王思令,郑子峥..SARS-CoV-2广谱中和抗体性质鉴定[J].中国免疫学杂志,2024,40(4):693-697,5.基金项目
国家自然科学基金项目(82071783). （82071783）
