植物学报2024，Vol.59Issue(2)：188-203,16.DOI:10.11983/CBB23107
金鱼草XTH家族基因鉴定及抗核盘菌和雄蕊瓣化相关基因筛选
Identification of XTH Family Genes in Antirrhinum majus and Screening of Genes Involoved in Sclerotinia sclerotiorum Resistance and Stamen Petalization
摘要
Abstract
Xyloglucan endotransglucosylase/hydrolases(XTH)belongs to glycoside hydrolase,family 16(GH16)and it is a class of enzymes that mediate the construction and recombination of xyglucan-cellulose skeleton.To explore the po-tential biological functions of XTH family genes in Antirrhinum majus.In this paper,bioinformatics analysis,RNA-seq analysis and qRT-PCR were used to investigate the expression levels of the family genes in the petalized and non-petalized stamens and disease-resistant materials.The results showed that the main conserved motif from 33 identi-fied AmXTH proteins was ExDxE,which could be divided into 3 subgroups.Most of the cis-acting elements of AmXTH promoter are growth and development,disease resistance and stress resistance.RNA-seq and qRT-PCR revalidation finally unearthed four positive candidate genes(AmXTH3,14,18,and 33)and one candidate genes(AmXTH23)for na-gatively mediated Sclerotinia sclerotiorum resistance.There were 12 positive AmXTH candidate genes(AmXTH1,7,9,11,21,22,23,24,26,28,29 and 33)and 2 negative AmXTH candidate genes(AmXTH15 and 31).Among them,AmXTH23 and 33 may play a role in both of the A.majus resistant to S.sclerotiorum and stamen petalization.In this study,the candidate AmXTH genes involved in S.sclerotiorum resistance and stamen petalization of A.majus were pre-liminarily excavated,which laid a foundation for further revealing the gene function.关键词
金鱼草/木葡聚糖内转糖苷酶/水解酶家族/核盘菌抗性/雄蕊瓣化/基因差异表达Key words
Antirrhinum majus/xyloglucan endotransglucosylase/hydrolases family/Sclerotinia sclerotiorum resistance/stamen petalization/differential gene expression引用本文复制引用
赵晗茜,宋佳怡,杨洁,赵永晶,夏文念,顾伟卓,汪仲毅,杨楠,胡慧贞..金鱼草XTH家族基因鉴定及抗核盘菌和雄蕊瓣化相关基因筛选[J].植物学报,2024,59(2):188-203,16.基金项目
国家自然科学基金地区科学基金(No.32360070)、国家自然科学基金青年基金(No.31901571)、西南林业大学科研启动项目(No.01102-112109)和云南省农业基础联合面上项目(No.202301BD070001-072) （No.32360070）
