植物学报2024，Vol.59Issue(2)：204-216,13.DOI:10.11983/CBB23109
蒙古冰草肉桂醇脱氢酶基因序列鉴定及功能分析
Sequence Identification and Functional Analysis of Cinnamyl Alcohol Dehydrogenase Gene from Agropyron mongolicum
摘要
Abstract
As an essential enzyme in plant secondary metabolism,cinnamyl alcohol dehydrogenase(CAD)plays a key role in regulating plant growth and development,as well as biological/abiotic stress resistance.Agropyron mongolicum is a traditional forage grass widely distributed in the desert grassland areas of northern China,which exhibited high tole-rance to drought and cold stresses.To explore the role of cinnamyl alcohol dehydrogenase in A.mongolicum,in this study,a CAD gene was identified from the full-length transcriptome data of A.mongolicum and subsequentially analyzed in vitro.The 1 083 bp coding sequence of AmCAD encodes 361 amino acids,which has typical conserved CAD region containing two Zn2+ binding motifs and NADP(H)cofactor binding motifs,belongs to the typical CAD protein,and its three-dimensional structure is similar to AtCAD5.AmCAD is highly expressed in the stem.The AmCAD recombinant protein showed a robust catalytic ability to different cinnamaldehyde substrates,with the highest substrate affinity of coniferyl aldehyde and sinapaldehyde.Under drought stress condition,the expression level of AmCAD was significantly induced,indicating a potential function of this gene in stress tolerance.The experimental results indicate that AmCAD may play an important role in lignin biosynthesis and drought stress tolerance in A.mongolicum.Our research provided potentially valuable genetic resources for molecular breeding of A.mongolicum to improve biomass quality and stress resistance.关键词
蒙古冰草/肉桂醇脱氢酶/木质素/抗旱性Key words
Agropyron mongolicum/cinnamyl alcohol dehydrogenase/lignin/drought resistance引用本文复制引用
王贺萍,刘亚玲,吴振映,何峰,付春祥,孙震,刘雨辰,苏彦龙,杜锦瑜,赵彦,赵竑博,王召明,苑峰..蒙古冰草肉桂醇脱氢酶基因序列鉴定及功能分析[J].植物学报,2024,59(2):204-216,13.基金项目
内蒙古自治区种业科技创新重大示范工程"揭榜挂帅"项目(No.2022JBGS0014)、中央引导地方科技发展资金(No.2022ZY006)、呼和浩特市科技创新领域人才项目("政产学研推用银"创新联合体项目)(No.2022RC-联合体-2)和山东能源研究院科研创新基金(No.SE11202142) （No.2022JBGS0014）
