植物学报2024，Vol.59Issue(2)：217-230,14.DOI:10.11983/CBB23161
水稻穗部性状QTL定位及候选基因分析
Mapping of QTLs Associated with Rice Panicle Traits and Candidate Gene Analysis
摘要
Abstract
Rice panicle traits have a distinct effect on yield,and the exploration and research of related genes play a crucial role in ensuring national food security.In this study,we used the indica rice HZ,the japonica rice Nekken2 and 120 recombinant inbred lines population constructed from them as experimental materials to measure panicle length,grain number per panicle,seed-setting rate,stigma exsertion rate,number of primary branches and other panicle traits.We combined the high-density genetic map for QTL mapping,a total of 31 QTLs were detected on chromosome 1,2,3,4,5,6,10 and 11,among which 2 LOD values were as high as 5.45 and 5.28.By analyzing the candidate genes in the QTL interval,the related genes that may affect the panicle traits were screened out,and qRT-PCR was used for gene expres-sion analysis,which revealed that the expression levels of LOC_Os05g05490,LOC_Os05g06150,LOC_Os03g11700,LOC_Os03g12430,LOC_Os05g28720,LOC_Os05g30890,LOC_Os05g31740 and LOC_Os02g17880 were significantly different between the parents.Among these genes,the first five genes encode tripartite pentapeptide repeat proteins,while the latter three genes encode glycosyltransferases.This study identified 31 QTLs related to panicle traits,laying a theoretical basis for further localization and cloning of related genes and breeding new high-yield rice varieties.关键词
水稻/穗部性状/QTL定位/候选基因/高产Key words
Oryza sativa(rice)/panicle traits/QTL mapping/candidate genes/high-yield引用本文复制引用
朱超宇,钟芊芊,殷文晶,王跃星,饶玉春,胡程翔,朱哲楠,张芷宁,汪理海,陈钧,李三峰,连锦瑾,唐璐瑶..水稻穗部性状QTL定位及候选基因分析[J].植物学报,2024,59(2):217-230,14.基金项目
浙江省自然科学基金(No.LZ23C130003)、2024年国家级大学生创新创业训练计划和2024年浙江省大学生科技创新活动计划暨新苗人才计划 （No.LZ23C130003）
