基于卵巢细胞外基质探索黄连对PCOS小鼠的治疗作用OACSTPCD

Objective:To explore the therapeutic effect of Rhizoma Coptidis on polycystic ovary syndrome(PCOS)mice based on ovarian extracellular matrix.Methods:Six C57BL/6 mice were selected as control group.Twenty-four C57BL/6 mice were subcutaneously injected with 21 d dehydroepiandrosterone(DHEA)to construct PCOS model.Six of them were in the model group(PCOS group),and the other 18 animals were treated with NaCl(PCOS + NaCl group),200 g·kg-1·d-1 active ingredient of Rhizoma Coptidis(PCOS + RC200 group)and 400 g·kg-1·d-1 active ingredient of Rhizoma Coptidis(PCOS + RC400 group)by gavage for 28 days,respectively.The expression levels of extracellular matrix remodeling related proteins MMP-2,COL-I and LOX-1 were detected by Western blot.Using KGN cells overetpressing and knocking down YTHDF2,mice were divided into PC3 1group,PC3 1-YTHDF2 group,and SIYTHDF2 group.Western blot was used to detect MMP-2 protein expression level.Results:Compared to control group,the levels of MMP-2,COL-I,and LOX-1 in the ovaries of PCOS group significantly increased(P<0.05).Compared to PCOS + NaCl group,the levels of MMP-2,COL-I,and LOX-1 in the ovaries of PCOS + RC200 group and PCOS + RC400 group significantly decreased(P<0.05).Compared to PC3.1 group,MMP-2,COL-I and LOX-1 levels significantly increased in PC3.1-YTHDF2 group(P<0.05).Compared to NC group,MMP-2 and COL-I levels significantly decreased in siYTHDF2 group(P<0.05).Conclusion:Rhizoma Coptidis may improve the extracellular matrix remodeling of PCOS ovaries by regulating MMP-2,COL-I,and LOX-1,thereby affecting ovulation.YTHDF2 can affect the expression levels of MMP-2,COL-I,and LOX-1 in KGN cells,and may be one of the targets for Rhizoma Coptidis treatment of PCOS.