福建农业学报2024，Vol.39Issue(2)：125-130,6.DOI:10.19303/j.issn.1008-0384.2024.02.001
一株具有插入和缺失突变特征鸭腺病毒B2毒株的分离鉴定
Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations
摘要
Abstract
[Objective]A previously unknown strain of duck adenovirus B2(DAdV B2)was isolated from a ducking of pale liver disease in Fujian and identified for epidemiological reference.[Methods]The virus associated with the disease was detected by PCR and isolated for identification by genome sequencing and a challenge test.[Result]The DAdV B2/BG48 virus infected LMH cells in the duck became swollen like a ballon,and eventually,died and disintegrated.The MDEF cells inoculated with the isolate changed from a spindle-shape to ball-like and clustered together with intercellular spaces in between.Distinctively,BG48 genome had a 3 bp insertion in the pX region,a 33 bp insertion in the ORF19B domain,and 42 bp deletion at the junction between ORF64 and ORF67.A G-base was found on the 133rd spot after the starting code of ORF67 and no truncated mutation in the terminal nor other codes compared with CH-GD-12-2014,or BG27 and BG18 previously identified.In a challenge test,BG48 induced a 50%morbidity and 0 mortality on 2-day-old Muscovy ducks with similar clinical symptoms and pathological changes between the artificially infected and the naturally diseased birds.[Conclusion]A novel DAdV B2 strain,BG48,from a Muscovy duck of pale liver disease was isolated and identified.The virus was uniquely characterized by multiple insertions and deletion mutations in the gene suggesting a potential of causing complex epidemic.关键词
番鸭白肝病/鸭腺病毒B2/分离鉴定/插入和缺失/ORF67Key words
Pale liver disease/duck adenovirus B2/isolation and identification/Insertion and deletion/ORF67分类
农业科技引用本文复制引用
郭博伦,李雅汝,郑欣,江丹丹,曾丽,陈少莺,马燕梅,陈仕龙..一株具有插入和缺失突变特征鸭腺病毒B2毒株的分离鉴定[J].福建农业学报,2024,39(2):125-130,6.基金项目
福建省科技计划公益类专项(2019R1026-11) （2019R1026-11）
福建省农业科学院科技创新团队建设项目(CXTD2021034) （CXTD2021034）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021018、XTCXGC2021012) （XTCXGC2021018、XTCXGC2021012）
福建农林大学科技创新专项基金(KFB23098A) （KFB23098A）
