海南中北部地区牛体表蜱种鉴定及蜱携带无形体属病原体情况调查研究OA北大核心CSTPCD
Identification of tick species on cattle in the central and northern regions of Hainan and investigation of tick-borne Anaplasma pathogens
目的:研究海南牛体表蜱虫的种类及蜱携带无形体属病原体的情况.方法:本研究选取海南中北部9个县市为样本采集点,从牛体表采集蜱虫样本.通过形态学观察和分子生物学技术进行蜱种鉴定,并以16S rRNA、groEL和gltA为靶基因,利用PCR方法检测蜱虫携带无形体属病原体情况.并进一步采用系统发育分析方法,揭示海南牛体表蜱虫携带的无形体属病原体种间关系.结果:本研究共采集335只蜱虫,包括血红扇头蜱(Rhipicephalus sanguineus)44只、微小扇头蜱(Rhipicephalus microplus)291只.PCR检测结果表明,该地区蜱中存在候选博莱无形体(Candidatus Anaplasma boleense)、边缘无形体(Anaplasma marginale),阳性率分别为0.60%(2/335),0.90%(3/335).系统发育分析结果表明,本实验获得的无形体与已知的无形体同源性较高.结论:海南中北部地区牛体表的优势蜱种为微小扇头蜱,并且蜱虫携带无形体属病原体,本研究为该地区无形体病的预防和治疗提供了重要的数据支撑.
Objective:To study the species of ticks found on cattle in Hainan and the prevalence of Anaplasma carried by these ticks.Methods:This study selected nine counties and cities in the central and northern parts of Hainan as sampling points to collect tick samples from cattle.Tick species were identified through morphological observation and molecular biological techniques.The presence of Anaplasma pathogens was detected using PCR with 16S rRNA,groEL,and gltA as target genes.Phylogenetic analy-sis methods were further employed to reveal the interspecies relationships of Anaplasma carried by ticks on cattle in Hainan.Re-sults:A total of 335 ticks were collected in this study,including 44 Rhipicephalus sanguineus and 291 Rhipicephalus microplus.PCR results indicated the presence of Candidatus Anaplasma boleense and Anaplasma marginale in the ticks,with positivity rates of 0.60%(2/335)and 0.90%(3/335),respectively.Phylogenetic analysis showed that the Anaplasma obtained in this experi-ment were homologous to known Anaplasma species.Conclusion:The dominant tick species on cattle in Hainan is Rhipicephalus microplus.The ticks were found to carry Anaplasma pathogens,and this study provides important data support for the prevention and treatment of Anaplasma diseases in the region.
邓宛心;李鲲;黄艺;彭箬岩;王高玉;贾毅博;牛毅;尹飞飞
海南医学院基础医学与生命科学院,海南 海口 571199||海南医学院-香港大学热带传染病联合实验室,海南 海口 571199中国疾病预防控制中心传染病预防控制所,北京 102206海南医学院-香港大学热带传染病联合实验室,海南 海口 571199
预防医学
微小扇头蜱无形体属病原体分子鉴定
Rhipicephalus microplusAnaplasma PathogensMolecular identification
《海南医学院学报》 2024 (009)
649-657 / 9
This study was supported by Key Research and Development Plan of Hainan Province(ZDYF2022SHFZ085);National Natural Science Foundation of China(82060378);Hainan Provincial Natural Science Foundation of China(822MS076,821QN0896),Graduate Innovation Project(HYYS2021A17,Qhys2021-352) 海南省重点研发计划项目(ZDYF2022SHFZ085);国家自然科学基金资助项目(82060378);海南省自然科学基金项目(822MS076,821QN0896);研究生创新课题(HYYS2021A17,Qhys2021-352)
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