海南医学院学报2024，Vol.30Issue(9)：649-657,9.DOI:10.13210/j.cnki.jhmu.20240220.003
海南中北部地区牛体表蜱种鉴定及蜱携带无形体属病原体情况调查研究
Identification of tick species on cattle in the central and northern regions of Hainan and investigation of tick-borne Anaplasma pathogens
摘要
Abstract
Objective:To study the species of ticks found on cattle in Hainan and the prevalence of Anaplasma carried by these ticks.Methods:This study selected nine counties and cities in the central and northern parts of Hainan as sampling points to collect tick samples from cattle.Tick species were identified through morphological observation and molecular biological techniques.The presence of Anaplasma pathogens was detected using PCR with 16S rRNA,groEL,and gltA as target genes.Phylogenetic analy-sis methods were further employed to reveal the interspecies relationships of Anaplasma carried by ticks on cattle in Hainan.Re-sults:A total of 335 ticks were collected in this study,including 44 Rhipicephalus sanguineus and 291 Rhipicephalus microplus.PCR results indicated the presence of Candidatus Anaplasma boleense and Anaplasma marginale in the ticks,with positivity rates of 0.60%(2/335)and 0.90%(3/335),respectively.Phylogenetic analysis showed that the Anaplasma obtained in this experi-ment were homologous to known Anaplasma species.Conclusion:The dominant tick species on cattle in Hainan is Rhipicephalus microplus.The ticks were found to carry Anaplasma pathogens,and this study provides important data support for the prevention and treatment of Anaplasma diseases in the region.关键词
微小扇头蜱/无形体属病原体/分子鉴定Key words
Rhipicephalus microplus/Anaplasma Pathogens/Molecular identification分类
医药卫生引用本文复制引用
邓宛心,李鲲,黄艺,彭箬岩,王高玉,贾毅博,牛毅,尹飞飞..海南中北部地区牛体表蜱种鉴定及蜱携带无形体属病原体情况调查研究[J].海南医学院学报,2024,30(9):649-657,9.基金项目
This study was supported by Key Research and Development Plan of Hainan Province(ZDYF2022SHFZ085) （ZDYF2022SHFZ085）
National Natural Science Foundation of China(82060378) （82060378）
Hainan Provincial Natural Science Foundation of China(822MS076,821QN0896),Graduate Innovation Project(HYYS2021A17,Qhys2021-352) 海南省重点研发计划项目(ZDYF2022SHFZ085) （822MS076,821QN0896）
国家自然科学基金资助项目(82060378) （82060378）
海南省自然科学基金项目(822MS076,821QN0896) （822MS076,821QN0896）
研究生创新课题(HYYS2021A17,Qhys2021-352) （HYYS2021A17,Qhys2021-352）
