海洋科学2024，Vol.48Issue(2)：32-42,11.DOI:10.11759/hykx20221109003
复方中草药对小丑鱼非特异性免疫、消化酶活性的影响及对刺激隐核虫杀灭效果分析
Effects of a Chinese herbal compound on nonspecific immu-nity,digestive enzyme activities,and extermination of the parasite Cryptocaryon irritans from Amphiprion frenatus
摘要
Abstract
"White spot disease"caused by Cryptocaryon irritans is harmful to cultured ornamental fish in a tank and also to marine ornamental fish living in large aquaria.It is one of the most serious diseases in marine ornamental fish farming.This study explored the effects of adding different doses of the compound Chinese herbal medicine XCY-1 to the basic feed on changes in nonspecific immune indicators in the serum and liver as well as digestive enzyme activities in the intestine of Am-phiprion frenatus.We also investigated the effects of XCY-1 for treating cryptocarioniasis.The results showed that the activities of SOD,LZM,AKP,ACP,GSH-Px,CAT,and T-AOC of clownfish increased first and then decreased after feeding the com-pound Chinese herbal medicine XCY-1.The indices reached their highest values on day 21.The immune ability of clownfish improved significantly in the 20 g/kg dose group.The activities of AMS,LPS,and trypsin in the intestinal tract were signifi-cantly higher than those in the control group.The activities of trypsin and AMS in the intestinal tract increased significantly in the 20 g/kg dose group,and the activities of LPS in the intestinal tract increased significantly in the 20 and 40 g/kg dose groups.The 50 mg/L XCY-1 dose had a killing effect on Cryptocaryon irritans larvae within 4 h,and all larvae were killed by 400 mg/L XCY-1.All larvae in the 800 mg/L dose group died within 3 h.A therapeutic experiment was performed in which fish were assigned randomly to groups A-C and a control.Fish in group A were fed 20 g/kg XCY-1 in the feed.Fish in group B were fed 20 g/kg XCY-1 in the feed and dipped in a 50 mg/L XCY-1 bath.Fish in group C were fed 20 g/kg XCY-1 in the feed and dipped in a 100 mg/L XCY-1 bath.The results showed that the survival rates of groups A,B,and C and the control group were 51.1%,53.3%,57.7%,and 0 on day 6.Only 0-2 trophonts were observed on each gill in groups A,B,and C,whereas the number of trophonts in the control group increased significantly.In conclusion,20 g/kg of the compound Chinese herbal medi-cine XCY-1 fed for 14-21 days improved serum and liver nonspecific immune indices and intestinal digestive enzyme activities in Amphiprion frenatus,XCY-1 also had a positive preventive and therapeutic effect on cryptocarioniasis.关键词
红小丑鱼/刺激隐核虫/中草药/非特异性免疫/消化酶Key words
Amphiprion frenatus/Cryptocaryon irritans/Chinese herbal compound/nonspecific immunity/digestive enzyme分类
农业科技引用本文复制引用
何丽斌,杨求华,李慧耀,罗辉玉,郑乐云..复方中草药对小丑鱼非特异性免疫、消化酶活性的影响及对刺激隐核虫杀灭效果分析[J].海洋科学,2024,48(2):32-42,11.基金项目
福建省属公益科研院所基本科研专项(2021R1013001) （2021R1013001）
福建省海洋与渔业结构调整专项(2019HYJG08)[Basic Research Project of Fujian Provincial Public Research Institute,No.2021R1013001 （2019HYJG08）
Marine and Fishery Structural Adjustment Project of Fujian Province,No.2019HYJG08] （）
