慢性牙周炎患者龈沟液中miR-498和miR-378h表达与Th17/Treg细胞平衡的关系OACSTPCD
The relationship between the expression of miR-498 and miR-378h in gingival crevicular fluid and Th17/Treg cell balance in patients with chronic periodontal disease
目的:探讨慢性牙周炎患者龈沟液中微小RNA-498(miR-498)、微小RNA-378h(miR-378h)表达与辅助性T细胞17(T helper cell 17,Th17)/调节性T细胞(regulatory T cell,Treg)细胞平衡的关系.方法:纳入我院2022 年1 月～2023 年 2 月期间收治的 97 例慢性牙周炎患者作为研究对象,根据病情严重程度分为轻度组(31 例)、中度组(42 例)和重度组(24 例),另选取同期在本院体检的 97 例牙周健康者作为对照组.测定分析慢性牙周炎患者龈沟液中miR-498 和miR-378h表达水平与Th17/Treg的相关性,探讨影响慢性牙周炎发生的危险因素并评估miR-498、miR-378h对慢性牙周炎的诊断价值.结果:研究组和对照组吸烟史、牙周指标、Th17、Treg、Th17/Treg比较,均存在显著差异(P<0.05);研究组龈沟液miR-498、miR-378h表达水平均高于对照组,且二者表达水平随慢性牙周炎严重程度的增加均呈现升高的趋势(P<0.05);龈沟液miR-498、miR-378h表达水平与血清Th17/Treg水平均呈正相关(r=0.669、0.803,P均<0.05).有吸烟史、Th17/Treg、miR-498 及miR-378h表达为发生慢性牙周炎的影响因素(P<0.05).龈沟液miR-498、miR-378h以及血清Th17/Treg单独诊断慢性牙周炎的AUC分别为 0.826、0.833、0.854,miR-498、miR-378h二者联合诊断的AUC为 0.932,高于miR-498、miR-378h以及Th17/Treg单独诊断(P<0.05).结论:龈沟液miR-498、miR-378h表达水平与血清Th17/Treg水平均呈正相关,且均与牙周炎的发生发展有关.
Objective:To investigate the relationship between the expression of microRNA-498(miR-498),microR-NA-378h(miR-378h)in gingival crevicular fluid(GCF)and the balance of T helper cell 17(Th17)/regulatory T cell(Treg)in patients with chronic periodontal disease.Methods:From January 2022 to February 2023,97 patients with chronic peri-odontitis admitted to our hospital were included as research subjects.They were grouped into mild group(31 cases),moderate group(42 cases)and severe group(24 cases)according to the severity of the disease,and 97 periodontally healthy subjects who were examined in our hospital were regarded as the control group.The correlation between the expression levels of miR-498 and miR-378h in gingival crevicular fluid of patients with chronic periodontitis and Th17/Treg were measured and ana-lyzed,exploring the risk factors affecting the occurrence of chronic periodontitis and evaluating the diagnostic value of miR-498 and miR-378h for chronic periodontitis.Results:There were obvious differences in smoking history,periodontal indica-tors,Th17,Treg,Th17/Treg between the study group and the control group(P<0.05).The expression levels of miR-498 and miR-378h in gingival crevicular fluid of the study group were higher than those of the control group(P<0.05),and the ex-pression levels of miR-498 and miR-378h in gingival crevicular fluid increased with the aggravation of chronic periodontal disease(P<0.05).The expression levels of miR-498 and miR-378h in gingival crevicular fluid were positively correlated with Th17/Treg(r=0.669,0.803,P<0.05).Smoking history,Th17/Treg,miR-498,and miR-378h were influencing factors for the occurrence of chronic periodontitis(P<0.05).The AUC of gingival crevicular fluid miR-498,miR-378h,and serum Th17/Treg for diagnosing chronic periodontitis alone were 0.826,0.833,and 0.854,respectively,the AUC of miR-498,miR-378h combined diagnosis was 0.932,which was higher than that of miR-498,miR-378h,and Th17/Treg alone diagnosis(P<0.05).Conclusion:The expression levels of miR-498 and miR-378h in gingival crevicular fluid are positively correlated with serum Th17/Treg levels,and are all related to the occurrence and development of periodontitis.
王惠;杜启雪;张若君;柏扬
广安门医院济南医院(济南市中医医院)口腔科 山东 济南 250012济南市第一人民医院五官科 山东 济南 250013
口腔医学
慢性牙周炎微小RNA-498微小RNA-378hTh17/Treg细胞平衡
Chronic periodontal diseaseMicro RNA-498Micro RNA-378hTh17/Treg cell balance
《临床口腔医学杂志》 2024 (005)
292-296 / 5
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