CTAGE15与食管鳞状细胞癌(ESCC)患者预后相关并抑制ESCC细胞的恶性表型OA北大核心CSTPCD
CTAGE15 correlates with prognosis in patients with esophageal squamous cell carcinoma(ESCC)and suppresses the malignant phenotype of ESCC cells
目的:探讨皮肤T细胞淋巴瘤相关抗原15(cutaneous T cell lymphoma associated antigen 15,CTAGE15)在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的表达与患者临床预后的关系及其对ESCC细胞增殖、迁移及侵袭等能力的影响.方法:Kaplan-Meier分析公共数据库中CTAGE15与ESCC患者预后的关系.qRT-PCR检测正常食管上皮细胞、ESCC细胞和临床120例随访信息完整的ESCC患者肿瘤组织及邻近正常组织中CTAGE15表达,COX生存风险分析方法研究ESCC患者的生存风险因素,多变量相关性分析CTAGE15表达与ESCC患者临床病理之间的关系,同时采用Kaplan-Meier分析CTAGE15对ESCC患者预后的影响.利用小干扰RNA沉默ESCC细胞中CTAGE15的表达,检测细胞增殖、迁移及侵袭能力的变化.结果:公共数据库中CTAGE15表达水平与患者总生存期(P＜0.05)和疾病特异性生存期(P＜0.05)显著相关,GTAGE15高表达患者生存期较好.qRT-PCR结果表明临床ESCC组织及ESCC细胞中CTAGE15表达显著高于正常食管上皮组织及正常食管上皮细胞,且CTAGE15表达水平与ESCC患者T分期与N分期显著相关(P＜0.05),CTAGE15高表达患者T分期、N分期较GTAGE15低表达患者早.敲低CTAGE15后,ESCC细胞的增殖、迁移及侵袭能力显著增强(P＜0.05).结论:CTAGE15与ESCC患者预后成正相关,且能够抑制ESCC细胞的增殖、迁移及侵袭等能力.
Objective:To investigate the expression of cutaneous T cell lymphoma associated antigen 15(CTAGE15)and its association with clinical prognosis of esophageal squamous cell carcinoma(ESCC)patients,as well as its effect on the proliferation,migration,and invasion abilities of ESCC cells.Methods:Kaplan-Meier analysis was used to analyze the effect of CTAGE15 on the prognosis of ESCC patients in public database.The mRNA expression levels of CTAGE15 in esophageal epithelial cells and ESCC cells were measured by qRT-PCR.Furthermore,the expression of CTAGE15 was detected in cancer and adjacent normal tissues from 120 ESCC patients with a complete follow-up information.COX proportional survival risk model was enrolled to analyze the survival risk factors of ESCC patients,and multivariate correlation analysis was used for the analysis of the association between CTAGE15 expression and the clinicopathology of ESCC patients.Kaplan-Meier analysis was employed to analyze the effect of CTAGE15 on the prognosis of ESCC patients.The knockdown of CTAGE15 in ESCC cells was achieved by using small interfering RNA(siRNA),and the changes in cell proliferation,migration,and invasion abilities of ESCC cells were assessed.Results:The public database showed that the CTAGE15 expression was significantly associated with overall survival(P＜0.05)and disease-specific survival(P＜0.05)in ESCC patients,and a higher GTAGE15 expression was correlated with a better survival.The qRT-PCR results revealed significantly high expression of CTAGE15 in ESCC tissues and cells,compared with normal esophageal epithelial tissues and cells.In addition,the expression of CTAGE15 was significantly associated with T stage and N stage of ESCC patients,and a higher GTAGE15 expression was associated with an earlier T stage and N stage.Knockdown of CTAGE15 enhanced the proliferation,migration,and invasion abilities of ESCC cells(P＜0.05).Conclusion:CTAGE15 expression is positively associated with the prognosis of ESCC patients,and it inhibits the proliferation,migration and invasion abilities of ESCC cells.
张博为;韩卓莹;马天云;赵珏莹;张莉;罗超;王其龙
南京医科大学附属淮安第一医院中心实验室,江苏 淮安 223300
临床医学
CTAGE15假基因食管鳞状细胞癌预后
CTAGE15pseudogenesesophageal squamous cell carcinomaprognosis
《南京医科大学学报（自然科学版）》 2024 (005)
634-642 / 9
江苏省自然科学基金(BK20211111);江苏省医学重点学科项目-肿瘤学(JSDW202233);江苏省研究生科研与实践创新计划项目(KYCX22_1806)
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